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2hz7

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{{STRUCTURE_2hz7| PDB=2hz7 | SCENE= }}
{{STRUCTURE_2hz7| PDB=2hz7 | SCENE= }}
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'''Crystal structure of the Glutaminyl-tRNA synthetase from Deinococcus radiodurans'''
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===Crystal structure of the Glutaminyl-tRNA synthetase from Deinococcus radiodurans===
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==Overview==
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Glutaminyl-tRNA synthetase from Deinococcus radiodurans possesses a C-terminal extension of 215 residues appending the anticodon-binding domain. This domain constitutes a paralog of the Yqey protein present in various organisms and part of it is present in the C-terminal end of the GatB subunit of GatCAB, a partner of the indirect pathway of Gln-tRNA(Gln) formation. To analyze the peculiarities of the structure-function relationship of this GlnRS related to the Yqey domain, a structure of the protein was solved from crystals diffracting at 2.3 A and a docking model of the synthetase complexed to tRNA(Gln) constructed. The comparison of the modeled complex with the structure of the E. coli complex reveals that all residues of E. coli GlnRS contacting tRNA(Gln) are conserved in D. radiodurans GlnRS, leaving the functional role of the Yqey domain puzzling. Kinetic investigations and tRNA-binding experiments of full length and Yqey-truncated GlnRSs reveal that the Yqey domain is involved in tRNA(Gln) recognition. They demonstrate that Yqey plays the role of an affinity-enhancer of GlnRS for tRNA(Gln) acting only in cis. However, the presence of Yqey in free state in organisms lacking GlnRS, suggests that this domain may exert additional cellular functions.
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(as it appears on PubMed at http://www.pubmed.gov), where 17284460 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17284460}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Deinococcus glutaminyl-tRNA synthetase is a chimer between proteins from an ancient and the modern pathways of aminoacyl-tRNA formation., Deniziak M, Sauter C, Becker HD, Paulus CA, Giege R, Kern D, Nucleic Acids Res. 2007;35(5):1421-31. Epub 2007 Feb 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17284460 17284460]
Deinococcus glutaminyl-tRNA synthetase is a chimer between proteins from an ancient and the modern pathways of aminoacyl-tRNA formation., Deniziak M, Sauter C, Becker HD, Paulus CA, Giege R, Kern D, Nucleic Acids Res. 2007;35(5):1421-31. Epub 2007 Feb 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17284460 17284460]
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Crystallization and preliminary X-ray characterization of the atypical glutaminyl-tRNA synthetase from Deinococcus radiodurans., Deniziak MA, Sauter C, Becker HD, Giege R, Kern D, Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 2):2361-3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15614972 15614972]
[[Category: Deinococcus radiodurans]]
[[Category: Deinococcus radiodurans]]
[[Category: Glutamine--tRNA ligase]]
[[Category: Glutamine--tRNA ligase]]
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[[Category: Glnrs core]]
[[Category: Glnrs core]]
[[Category: Rossmann fold]]
[[Category: Rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:53:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:33:05 2008''

Revision as of 18:33, 28 July 2008

Image:2hz7.png

Template:STRUCTURE 2hz7

Crystal structure of the Glutaminyl-tRNA synthetase from Deinococcus radiodurans

Template:ABSTRACT PUBMED 17284460

About this Structure

2HZ7 is a Single protein structure of sequence from Deinococcus radiodurans. Full crystallographic information is available from OCA.

Reference

Deinococcus glutaminyl-tRNA synthetase is a chimer between proteins from an ancient and the modern pathways of aminoacyl-tRNA formation., Deniziak M, Sauter C, Becker HD, Paulus CA, Giege R, Kern D, Nucleic Acids Res. 2007;35(5):1421-31. Epub 2007 Feb 6. PMID:17284460

Crystallization and preliminary X-ray characterization of the atypical glutaminyl-tRNA synthetase from Deinococcus radiodurans., Deniziak MA, Sauter C, Becker HD, Giege R, Kern D, Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 2):2361-3. PMID:15614972

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