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| - | [[Image:2i0i.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2i0i| PDB=2i0i | SCENE= }} | | {{STRUCTURE_2i0i| PDB=2i0i | SCENE= }} |
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| - | '''X-ray crystal structure of Sap97 PDZ3 bound to the C-terminal peptide of HPV18 E6'''
| + | ===X-ray crystal structure of Sap97 PDZ3 bound to the C-terminal peptide of HPV18 E6=== |
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| - | ==Overview==
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| - | Human papillomavirus (HPV) E6 oncoprotein targets certain tumor suppressors such as MAGI-1 and SAP97/hDlg for degradation. A short peptide at the C terminus of E6 interacts specifically with the PDZ domains of these tumor suppressors, which is a property unique to high-risk HPVs that are associated with cervical cancer. The detailed recognition mechanisms between HPV E6 and PDZ proteins are unclear. To understand the specific binding of cellular PDZ substrates by HPV E6, we have solved the crystal structures of the complexes containing a peptide from HPV18 E6 bound to three PDZ domains from MAGI-1 and SAP97/Dlg. The complex crystal structures reveal novel features of PDZ peptide recognition that explain why high-risk HPV E6 can specifically target these cellular tumor suppressors for destruction. Moreover, a new peptide-binding loop on these PDZs is identified as interacting with the E6 peptide. Furthermore, we have identified an arginine residue, unique to high-risk HPV E6 but outside the canonical core PDZ recognition motif, that plays an important role in the binding of the PDZs of both MAGI-I and SAP97/Dlg, the mutation of which abolishes E6's ability to degrade the two proteins. Finally, we have identified a dimer form of MAGI-1 PDZ domain 1 in the cocrystal structure with E6 peptide, which may have functional relevance for MAGI-1 activity. In addition to its novel insights into the biochemistry of PDZ interactions, this study is important for understanding HPV-induced oncogenesis; this could provide a basis for developing antiviral and anticancer compounds.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17267502}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17267502 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17267502}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Sap97 pdz3]] | | [[Category: Sap97 pdz3]] |
| | [[Category: Tumor suppressor]] | | [[Category: Tumor suppressor]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:55:52 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:53:13 2008'' |
Revision as of 13:53, 27 July 2008
Template:STRUCTURE 2i0i
X-ray crystal structure of Sap97 PDZ3 bound to the C-terminal peptide of HPV18 E6
Template:ABSTRACT PUBMED 17267502
About this Structure
2I0I is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structures of a human papillomavirus (HPV) E6 polypeptide bound to MAGUK proteins: mechanisms of targeting tumor suppressors by a high-risk HPV oncoprotein., Zhang Y, Dasgupta J, Ma RZ, Banks L, Thomas M, Chen XS, J Virol. 2007 Apr;81(7):3618-26. Epub 2007 Jan 31. PMID:17267502
Page seeded by OCA on Sun Jul 27 16:53:13 2008
