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2i1t

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[[Image:2i1t.gif|left|200px]]
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[[Image:2i1t.png|left|200px]]
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{{STRUCTURE_2i1t| PDB=2i1t | SCENE= }}
{{STRUCTURE_2i1t| PDB=2i1t | SCENE= }}
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'''Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels'''
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===Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels===
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{{ABSTRACT_PUBMED_15084603}}
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==Overview==
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We have isolated a cardiotoxin, denoted jingzhaotoxin-III (JZTX-III), from the venom of the Chinese spider Chilobrachys jingzhao. The toxin contains 36 residues stabilized by three intracellular disulfide bridges (I-IV, II-V, and III-VI), assigned by a chemical strategy of partial reduction and sequence analysis. Cloned and sequenced using 3'-rapid amplification of cDNA ends and 5'-rapid amplification of cDNA ends, the full-length cDNA encoded a 63-residue precursor of JZTX-III. Different from other spider peptides, it contains an uncommon endoproteolytic site (-X-Ser-) anterior to mature protein and the intervening regions of 5 residues, which is the smallest in spider toxin cDNAs identified to date. Under whole cell recording, JZTX-III showed no effects on voltage-gated sodium channels (VGSCs) or calcium channels in dorsal root ganglion neurons, whereas it significantly inhibited tetrodotoxin-resistant VGSCs with an IC(50) value of 0.38 microm in rat cardiac myocytes. Different from scorpion beta-toxins, it caused a 10-mV depolarizing shift in the channel activation threshold. The binding site for JZTX-III on VGSCs is further suggested to be site 4 with a simple competitive assay, which at 10 microm eliminated the slowing currents induced by Buthus martensi Karsch I (BMK-I, scorpion alpha-like toxin) completely. JZTX-III shows higher selectivity for VGSC isoforms than other spider toxins affecting VGSCs, and the toxin hopefully represents an important ligand for discriminating cardiac VGSC subtype.
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==About this Structure==
==About this Structure==
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2I1T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chilobrachys_jingzhao Chilobrachys jingzhao]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA].
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[[2i1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chilobrachys_guangxiensis Chilobrachys guangxiensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA].
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[[Category: Chilobrachys guangxiensis]]
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==Reference==
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Jingzhaotoxin-III, a novel spider toxin inhibiting activation of voltage-gated sodium channel in rat cardiac myocytes., Xiao Y, Tang J, Yang Y, Wang M, Hu W, Xie J, Zeng X, Liang S, J Biol Chem. 2004 Jun 18;279(25):26220-6. Epub 2004 Apr 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15084603 15084603]
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[[Category: Chilobrachys jingzhao]]
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[[Category: Single protein]]
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[[Category: Liang, S.]]
[[Category: Liang, S.]]
[[Category: Liao, Z.]]
[[Category: Liao, Z.]]
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[[Category: Nav channel]]
[[Category: Nav channel]]
[[Category: Solution structure]]
[[Category: Solution structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:58:33 2008''
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[[Category: Toxin]]

Revision as of 08:00, 24 October 2012

Image:2i1t.png

Template:STRUCTURE 2i1t

Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels

Template:ABSTRACT PUBMED 15084603

About this Structure

2i1t is a 1 chain structure with sequence from Chilobrachys guangxiensis. Full experimental information is available from OCA.

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OCA

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