2i3b

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[[Image:2i3b.jpg|left|200px]]
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{{STRUCTURE_2i3b| PDB=2i3b | SCENE= }}
{{STRUCTURE_2i3b| PDB=2i3b | SCENE= }}
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'''Solution Structure of a Human Cancer-Related Nucleoside Triphosphatase'''
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===Solution Structure of a Human Cancer-Related Nucleoside Triphosphatase===
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==Overview==
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A screen of the human cancer genome anatomy project (CGAP) database was performed to search for new proteins involved in tumorigenesis. The resulting hits were further screened for recombinant expression, solubility and protein aggregation, which led to the identification of the previously unknown human cancer-related (HCR) protein encoded by the mRNA NM_032324 as a target for structure determination by NMR. The three-dimensional structure of the protein in its complex with ATPgammaS forms a three-layered alpha/beta sandwich, with a central nine-stranded beta-sheet surrounded by five alpha-helices. Sequence and three-dimensional structure comparisons with AAA+ ATPases revealed the presence of Walker A (GPPGVGKT) and Walker B (VCVIDEIG) motifs. Using 1D (31)P-NMR spectroscopy and a coupled enzymatic assay for the determination of inorganic phosphate, we showed that the purified recombinant protein is active as a non-specific nucleoside triphosphatase, with k(cat)=7.6x10(-3) s(-1). The structural basis for the enzymatic activity of HCR-NTPase was further characterized by site-directed mutagenesis of the Walker B motif, which further contributes to making the HCR-NTPase an attractive new target for further biochemical characterization in the context of its presumed role in human tumorigenesis.
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(as it appears on PubMed at http://www.pubmed.gov), where 17291528 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17291528}}
==About this Structure==
==About this Structure==
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2I3B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I3B OCA].
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2I3B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I3B OCA].
==Reference==
==Reference==
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[[Category: Ntpase]]
[[Category: Ntpase]]
[[Category: Rossmann]]
[[Category: Rossmann]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:01:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:58:17 2008''

Revision as of 01:58, 29 July 2008

Image:2i3b.png

Template:STRUCTURE 2i3b

Solution Structure of a Human Cancer-Related Nucleoside Triphosphatase

Template:ABSTRACT PUBMED 17291528

About this Structure

2I3B is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

NMR structure and functional characterization of a human cancer-related nucleoside triphosphatase., Placzek WJ, Almeida MS, Wuthrich K, J Mol Biol. 2007 Mar 30;367(3):788-801. Epub 2007 Jan 9. PMID:17291528

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