2i5p

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{{STRUCTURE_2i5p| PDB=2i5p | SCENE= }}
{{STRUCTURE_2i5p| PDB=2i5p | SCENE= }}
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'''Crystal structure of glyceraldehyde-3-phosphate dehydrogenase isoform 1 from K. marxianus'''
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===Crystal structure of glyceraldehyde-3-phosphate dehydrogenase isoform 1 from K. marxianus===
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==Overview==
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The presence of an isoform of glyceraldehyde-3-phosphate dehydrogenase (kmGAPDH1p) associated with the cell wall of a flocculent strain of Kluyveromyces marxianus was the first report of a non-cytosolic localization of a glycolytic enzyme, but the mechanism by which the protein is transported to the cell surface is not known. To identify structural features that could account for the multiple localizations of the protein, the three-dimensional structure of kmGAPDH1p was determined by x-ray crystallography and small angle x-ray scattering. The x-ray crystallographic structure of kmGAPDH1p revealed a dimer, although all GAPDH homologs studied thus far have a tetrameric structure with 222 symmetry. Interestingly, the structure of kmGAPDH1p in solution revealed a tetramer with a 70 degrees tilt angle between the dimers. Moreover, the separation between the centers of the dimers composing the kmGAPDH1p tetramer diminished from 34 to 30 A upon NAD(+) binding, this latter value being similar to the observed in the crystallographic models of GAPDH homologs. The less compact structure of apo-kmGAPDH1p could already be the first image of the transition intermediate between the tetramer observed in solution and the dimeric form found in the crystal structure, which we postulate to exist in vivo because of the protein's multiple subcellular localizations in this yeast species.
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{{ABSTRACT_PUBMED_16963457}}
==About this Structure==
==About this Structure==
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[[Category: Gapdh]]
[[Category: Gapdh]]
[[Category: Rossmann fold]]
[[Category: Rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:06:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:28:02 2008''

Revision as of 11:28, 28 July 2008

Image:2i5p.png

Template:STRUCTURE 2i5p

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase isoform 1 from K. marxianus

Template:ABSTRACT PUBMED 16963457

About this Structure

2I5P is a Single protein structure of sequence from Kluyveromyces marxianus. Full crystallographic information is available from OCA.

Reference

The crystal and solution structures of glyceraldehyde-3-phosphate dehydrogenase reveal different quaternary structures., Ferreira-da-Silva F, Pereira PJ, Gales L, Roessle M, Svergun DI, Moradas-Ferreira P, Damas AM, J Biol Chem. 2006 Nov 3;281(44):33433-40. Epub 2006 Sep 7. PMID:16963457

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