2qg6
From Proteopedia
(New page: 200px<br /> <applet load="2qg6" size="450" color="white" frame="true" align="right" spinBox="true" caption="2qg6, resolution 1.500Å" /> '''Crystal structure ...) |
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caption="2qg6, resolution 1.500Å" /> | caption="2qg6, resolution 1.500Å" /> | ||
'''Crystal structure of human nicotinamide riboside kinase (NRK1) in complex with nicotinamide mononucleotide (NMN)'''<br /> | '''Crystal structure of human nicotinamide riboside kinase (NRK1) in complex with nicotinamide mononucleotide (NMN)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2QG6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with PO4 and NMN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2QG6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=NMN:'>NMN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:21:10 2008'' |
Revision as of 11:21, 23 January 2008
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Crystal structure of human nicotinamide riboside kinase (NRK1) in complex with nicotinamide mononucleotide (NMN)
Overview
Nicotinamide riboside kinase (NRK) has an important role in the, biosynthesis of NAD(+) as well as the activation of tiazofurin and other, NR analogs for anticancer therapy. NRK belongs to the deoxynucleoside, kinase and nucleoside monophosphate (NMP) kinase superfamily, although the, degree of sequence conservation is very low. We report here the crystal, structures of human NRK1 in a binary complex with the reaction product, nicotinamide mononucleotide (NMN) at 1.5 A resolution and in a ternary, complex with ADP and tiazofurin at 2.7 A resolution. The active site is, located in a groove between the central parallel beta sheet core and the, LID and NMP-binding domains. The hydroxyl groups on the ribose of NR are, recognized by Asp56 and Arg129, and Asp36 is the general base of the, enzyme. Mutation of residues in the active site can abolish the catalytic, activity of the enzyme, confirming the structural observations.
About this Structure
2QG6 is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of human nicotinamide riboside kinase., Khan JA, Xiang S, Tong L, Structure. 2007 Aug;15(8):1005-13. PMID:17698003
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