2ig0

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{{STRUCTURE_2ig0| PDB=2ig0 | SCENE= }}
{{STRUCTURE_2ig0| PDB=2ig0 | SCENE= }}
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'''Structure of 53BP1/methylated histone peptide complex'''
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===Structure of 53BP1/methylated histone peptide complex===
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==Overview==
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Histone lysine methylation has been linked to the recruitment of mammalian DNA repair factor 53BP1 and putative fission yeast homolog Crb2 to DNA double-strand breaks (DSBs), but how histone recognition is achieved has not been established. Here we demonstrate that this link occurs through direct binding of 53BP1 and Crb2 to histone H4. Using X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy, we show that, despite low amino acid sequence conservation, both 53BP1 and Crb2 contain tandem tudor domains that interact with histone H4 specifically dimethylated at Lys20 (H4-K20me2). The structure of 53BP1/H4-K20me2 complex uncovers a unique five-residue 53BP1 binding cage, remarkably conserved in the structure of Crb2, that best accommodates a dimethyllysine but excludes a trimethyllysine, thus explaining the methylation state-specific recognition of H4-K20. This study reveals an evolutionarily conserved molecular mechanism of targeting DNA repair proteins to DSBs by direct recognition of H4-K20me2.
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{{ABSTRACT_PUBMED_17190600}}
==About this Structure==
==About this Structure==
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[[Category: Dna repair]]
[[Category: Dna repair]]
[[Category: Tandem tudor domain]]
[[Category: Tandem tudor domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:27:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:54:01 2008''

Revision as of 01:54, 29 July 2008

Image:2ig0.png

Template:STRUCTURE 2ig0

Structure of 53BP1/methylated histone peptide complex

Template:ABSTRACT PUBMED 17190600

About this Structure

2IG0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair., Botuyan MV, Lee J, Ward IM, Kim JE, Thompson JR, Chen J, Mer G, Cell. 2006 Dec 29;127(7):1361-73. PMID:17190600

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