2ih0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2ih0.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2ih0.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2ih0| PDB=2ih0 | SCENE= }}
{{STRUCTURE_2ih0| PDB=2ih0 | SCENE= }}
-
'''NMR structure determination of a synthetic analogue of the iturinic antibiotic bacillomycin Lc'''
+
===NMR structure determination of a synthetic analogue of the iturinic antibiotic bacillomycin Lc===
-
==Overview==
+
<!--
-
Iturins are a group of antifungal produced by Bacillus subtilis. All are cyclic lipopeptides with seven alpha-amino acids of configuration LDDLLDL and one beta-amino fatty acid. The bacillomycin L is a member of this family and its NMR structure was previously resolved using the sequence Asp-Tyr-Asn-Ser-Gln-Ser-Thr. In this work, we carefully examined the NMR spectra of this compound and detected an error in the sequence. In fact, Asp1 and Gln5 need to be changed into Asn1 and Glu5, which therefore makes it identical to bacillomycin Lc. As a consequence, it now appears that all iturinic peptides with antibiotic activity share the common beta-amino fatty acid 8-L-Asn1-D-Tyr2-D-Asn3 sequence. To better understand the conformational influence of the acidic residue L-Asp1, present, for example in the inactive iturin C, the NMR structure of the synthetic analogue SCP [cyclo (L-Asp1-D-Tyr2-D-Asn3-L-Ser4-L-Gln5-D-Ser6-L-Thr7-beta-Ala8)] was determined and compared with bacillomycin Lc recalculated with the corrected sequence. In both cases, the conformers obtained were separated into two families of similar energy which essentially differ in the number and type of turns. A detailed analysis of both cyclopeptide structures is presented here. In addition, CD and FTIR spectra were performed and confirmed the conformational differences observed by NMR between both cyclopeptides.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17129757}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17129757 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17129757}}
==About this Structure==
==About this Structure==
-
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IH0 OCA].
+
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IH0 OCA].
==Reference==
==Reference==
NMR structure determination of a synthetic analogue of bacillomycin Lc reveals the strategic role of L-Asn1 in the natural iturinic antibiotics., Volpon L, Tsan P, Majer Z, Vass E, Hollosi M, Noguera V, Lancelin JM, Besson F, Spectrochim Acta A Mol Biomol Spectrosc. 2007 Aug;67(5):1374-81. Epub 2006, Oct 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17129757 17129757]
NMR structure determination of a synthetic analogue of bacillomycin Lc reveals the strategic role of L-Asn1 in the natural iturinic antibiotics., Volpon L, Tsan P, Majer Z, Vass E, Hollosi M, Noguera V, Lancelin JM, Besson F, Spectrochim Acta A Mol Biomol Spectrosc. 2007 Aug;67(5):1374-81. Epub 2006, Oct 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17129757 17129757]
 +
 +
NMR structure of active and inactive forms of the sterol-dependent antifungal antibiotic bacillomycin L., Volpon L, Besson F, Lancelin JM, Eur J Biochem. 1999 Aug;264(1):200-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10447689 10447689]
[[Category: Besson, F.]]
[[Category: Besson, F.]]
[[Category: Lancelin, J.]]
[[Category: Lancelin, J.]]
Line 28: Line 34:
[[Category: Iturin]]
[[Category: Iturin]]
[[Category: Synthetic peptide]]
[[Category: Synthetic peptide]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:29:33 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:43:36 2008''

Revision as of 09:43, 29 July 2008

Image:2ih0.png

Template:STRUCTURE 2ih0

NMR structure determination of a synthetic analogue of the iturinic antibiotic bacillomycin Lc

Template:ABSTRACT PUBMED 17129757

About this Structure

Full experimental information is available from OCA.

Reference

NMR structure determination of a synthetic analogue of bacillomycin Lc reveals the strategic role of L-Asn1 in the natural iturinic antibiotics., Volpon L, Tsan P, Majer Z, Vass E, Hollosi M, Noguera V, Lancelin JM, Besson F, Spectrochim Acta A Mol Biomol Spectrosc. 2007 Aug;67(5):1374-81. Epub 2006, Oct 19. PMID:17129757

NMR structure of active and inactive forms of the sterol-dependent antifungal antibiotic bacillomycin L., Volpon L, Besson F, Lancelin JM, Eur J Biochem. 1999 Aug;264(1):200-10. PMID:10447689

Page seeded by OCA on Tue Jul 29 12:43:36 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ih0"
Personal tools