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2ihn

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[[Image:2ihn.gif|left|200px]]
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{{STRUCTURE_2ihn| PDB=2ihn | SCENE= }}
{{STRUCTURE_2ihn| PDB=2ihn | SCENE= }}
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'''Co-crystal of Bacteriophage T4 RNase H with a fork DNA substrate'''
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===Co-crystal of Bacteriophage T4 RNase H with a fork DNA substrate===
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==Overview==
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Bacteriophage T4 RNase H, a flap endonuclease-1 family nuclease, removes RNA primers from lagging strand fragments. It has both 5' nuclease and flap endonuclease activities. Our previous structure of native T4 RNase H (PDB code 1TFR) revealed an active site composed of highly conserved Asp residues and two bound hydrated magnesium ions. Here, we report the crystal structure of T4 RNase H in complex with a fork DNA substrate bound in its active site. This is the first structure of a flap endonuclease-1 family protein with its complete branched substrate. The fork duplex interacts with an extended loop of the helix-hairpin-helix motif class 2. The 5' arm crosses over the active site, extending below the bridge (helical arch) region. Cleavage assays of this DNA substrate identify a primary cut site 7-bases in from the 5' arm. The scissile phosphate, the first bond in the duplex DNA adjacent to the 5' arm, lies above a magnesium binding site. The less ordered 3' arm reaches toward the C and N termini of the enzyme, which are binding sites for T4 32 protein and T4 45 clamp, respectively. In the crystal structure, the scissile bond is located within the double-stranded DNA, between the first two duplex nucleotides next to the 5' arm, and lies above a magnesium binding site. This complex provides important insight into substrate recognition and specificity of the flap endonuclease-1 enzymes.
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{{ABSTRACT_PUBMED_17693399}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase/dna complex]]
[[Category: Hydrolase/dna complex]]
[[Category: Protein:dna complex]]
[[Category: Protein:dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:30:58 2008''
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Revision as of 08:30, 29 July 2008

Image:2ihn.png

Template:STRUCTURE 2ihn

Co-crystal of Bacteriophage T4 RNase H with a fork DNA substrate

Template:ABSTRACT PUBMED 17693399

About this Structure

2IHN is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Crystal structure of bacteriophage T4 5' nuclease in complex with a branched DNA reveals how flap endonuclease-1 family nucleases bind their substrates., Devos JM, Tomanicek SJ, Jones CE, Nossal NG, Mueser TC, J Biol Chem. 2007 Oct 26;282(43):31713-24. Epub 2007 Aug 9. PMID:17693399

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