2is2

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[[Image:2is2.gif|left|200px]]
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{{STRUCTURE_2is2| PDB=2is2 | SCENE= }}
{{STRUCTURE_2is2| PDB=2is2 | SCENE= }}
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'''Crystal structure of UvrD-DNA binary complex'''
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===Crystal structure of UvrD-DNA binary complex===
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==Overview==
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Helicases use the energy derived from nucleoside triphosphate hydrolysis to unwind double helices in essentially every metabolic pathway involving nucleic acids. Earlier crystal structures have suggested that DNA helicases translocate along a single-stranded DNA in an inchworm fashion. We report here a series of crystal structures of the UvrD helicase complexed with DNA and ATP hydrolysis intermediates. These structures reveal that ATP binding alone leads to unwinding of 1 base pair by directional rotation and translation of the DNA duplex, and ADP and Pi release leads to translocation of the developing single strand. Thus DNA unwinding is achieved by a two-part power stroke in a combined wrench-and-inchworm mechanism. The rotational angle and translational distance of DNA define the unwinding step to be 1 base pair per ATP hydrolyzed. Finally, a gateway for ssDNA translocation and an alternative strand-displacement mode may explain the varying step sizes reported previously.
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(as it appears on PubMed at http://www.pubmed.gov), where 17190599 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17190599}}
==About this Structure==
==About this Structure==
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[[Category: Yang, W.]]
[[Category: Yang, W.]]
[[Category: Dna helicase]]
[[Category: Dna helicase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:47:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:32:09 2008''

Revision as of 18:32, 28 July 2008

Image:2is2.png

Template:STRUCTURE 2is2

Crystal structure of UvrD-DNA binary complex

Template:ABSTRACT PUBMED 17190599

About this Structure

2IS2 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke., Lee JY, Yang W, Cell. 2006 Dec 29;127(7):1349-60. PMID:17190599

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