2isl
From Proteopedia
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{{STRUCTURE_2isl| PDB=2isl | SCENE= }} | {{STRUCTURE_2isl| PDB=2isl | SCENE= }} | ||
| - | + | ===BluB bound to reduced flavin (FMNH2) and molecular oxygen. (clear crystal form)=== | |
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| - | ==Overview== | ||
| - | An insight into a previously unknown step in B(12) biosynthesis was unexpectedly obtained through our analysis of a mutant of the symbiotic nitrogen fixing bacterium Sinorhizobium meliloti. This mutant was identified based on its unusually bright fluorescence on plates containing the succinoglycan binding dye calcofluor. The mutant contains a Tn5 insertion in a gene that has not been characterized previously in S. meliloti. The closest known homolog is the bluB gene of Rhodobacter capsulatus, which is implicated in the biosynthesis of B(12) (cobalamin). The S. meliloti bluB mutant is unable to grow in minimal media and fails to establish a symbiosis with alfalfa, and these defects can be rescued by the addition of vitamin B(12) (cyanocobalamin) or the lower ligand of cobalamin, 5,6-dimethylbenzimidazole (DMB). Biochemical analysis demonstrated that the bluB mutant does not produce cobalamin unless DMB is supplied. Sequence comparison suggests that BluB is a member of the NADH/flavin mononucleotide (FMN)-dependent nitroreductase family, and we propose that it is involved in the conversion of FMN to DMB. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2ISL is a | + | 2ISL is a 8 chains structure of sequences from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ISL OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:16537439</ref><references group="xtra"/> | |
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[[Category: Sinorhizobium meliloti]] | [[Category: Sinorhizobium meliloti]] | ||
[[Category: Howard-Jones, A R.]] | [[Category: Howard-Jones, A R.]] | ||
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[[Category: Monooxygenase]] | [[Category: Monooxygenase]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
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| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:50:36 2009'' | ||
Revision as of 16:50, 17 February 2009
BluB bound to reduced flavin (FMNH2) and molecular oxygen. (clear crystal form)
About this Structure
2ISL is a 8 chains structure of sequences from Sinorhizobium meliloti. Full crystallographic information is available from OCA.
Reference
- Campbell GR, Taga ME, Mistry K, Lloret J, Anderson PJ, Roth JR, Walker GC. Sinorhizobium meliloti bluB is necessary for production of 5,6-dimethylbenzimidazole, the lower ligand of B12. Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4634-9. Epub 2006 Mar 1. PMID:16537439
Page seeded by OCA on Tue Feb 17 18:50:36 2009
