2itj

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{{STRUCTURE_2itj| PDB=2itj | SCENE= }}
{{STRUCTURE_2itj| PDB=2itj | SCENE= }}
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'''Origin binding domain of the SV40 large T antigen (residues 131-259). P212121 crystal form'''
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===Origin binding domain of the SV40 large T antigen (residues 131-259). P212121 crystal form===
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==Overview==
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The large T antigen (T-ag) protein binds to and activates DNA replication from the origin of DNA replication (ori) in simian virus 40 (SV40). Here, we determined the crystal structures of the T-ag origin-binding domain (OBD) in apo form, and bound to either a 17 bp palindrome (sites 1 and 3) or a 23 bp ori DNA palindrome comprising all four GAGGC binding sites for OBD. The T-ag OBDs were shown to interact with the DNA through a loop comprising Ser147-Thr155 (A1 loop), a combination of a DNA-binding helix and loop (His203-Asn210), and Asn227. The A1 loop traveled back-and-forth along the major groove and accounted for most of the sequence-determining contacts with the DNA. Unexpectedly, in both T-ag-DNA structures, the T-ag OBDs bound DNA independently and did not make direct protein-protein contacts. The T-ag OBD was also captured bound to a non-consensus site ATGGC even in the presence of its canonical site GAGGC. Our observations taken together with the known biochemical and structural features of the T-ag-origin interaction suggest a model for origin unwinding.
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{{ABSTRACT_PUBMED_17139255}}
==About this Structure==
==About this Structure==
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[[Category: Martynowski, D.]]
[[Category: Martynowski, D.]]
[[Category: Dna binding protein]]
[[Category: Dna binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:55:05 2008''

Revision as of 08:55, 29 July 2008

Image:2itj.png

Template:STRUCTURE 2itj

Origin binding domain of the SV40 large T antigen (residues 131-259). P212121 crystal form

Template:ABSTRACT PUBMED 17139255

About this Structure

2ITJ is a Single protein structure of sequence from Simian virus 40. Full crystallographic information is available from OCA.

Reference

Structure of the origin-binding domain of simian virus 40 large T antigen bound to DNA., Bochkareva E, Martynowski D, Seitova A, Bochkarev A, EMBO J. 2006 Dec 13;25(24):5961-9. Epub 2006 Nov 30. PMID:17139255

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