2iwm

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{{STRUCTURE_2iwm| PDB=2iwm | SCENE= }}
{{STRUCTURE_2iwm| PDB=2iwm | SCENE= }}
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'''PRECURSOR MUTANT CYS1SER OF PENICILLIN V ACYLASE FROM BACILLUS SPHAERICUS'''
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===PRECURSOR MUTANT CYS1SER OF PENICILLIN V ACYLASE FROM BACILLUS SPHAERICUS===
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==Overview==
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The crystallization of three catalytically inactive mutants of penicillin V acylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzyme. Directed mutants and clone constructs were designed to study the post-translational autoproteolytic processing of PVA. The catalytically inactive mutants will provide three-dimensional structures of precursor PVA forms, plus open a route to the study of enzyme-substrate complexes for this industrially important enzyme.
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{{ABSTRACT_PUBMED_16508111}}
==About this Structure==
==About this Structure==
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[[Category: Precursor]]
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[[Category: Zymogen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:15:41 2008''

Revision as of 11:15, 28 July 2008

Image:2iwm.png

Template:STRUCTURE 2iwm

PRECURSOR MUTANT CYS1SER OF PENICILLIN V ACYLASE FROM BACILLUS SPHAERICUS

Template:ABSTRACT PUBMED 16508111

About this Structure

2IWM is a Single protein structure of sequence from Lysinibacillus sphaericus. Full crystallographic information is available from OCA.

Reference

Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:16508111

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