This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.




2j32

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2j32.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2j32.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2j32| PDB=2j32 | SCENE= }}
{{STRUCTURE_2j32| PDB=2j32 | SCENE= }}
-
'''THE ROLE OF LOOP BUNDLE HYDROGEN BONDS IN THE MATURATION AND ACTIVITY OF(PRO)CASPASE-3'''
+
===THE ROLE OF LOOP BUNDLE HYDROGEN BONDS IN THE MATURATION AND ACTIVITY OF(PRO)CASPASE-3===
-
==Overview==
+
<!--
-
During maturation, procaspase-3 is cleaved at D175, which resides in a linker that connects the large and small subunits. The intersubunit linker also connects two active site loops that rearrange following cleavage and, in part, form the so-called loop bundle. As a result of chain cleavage, new hydrogen bonds and van der Waals contacts form among three active site loops. The new interactions are predicted to stabilize the active site. One unresolved issue is the extent to which the loop bundle residues also stabilize the procaspase active site. We examined the effects of replacing four loop bundle residues (E167, D169, E173, and Y203) on the biochemical and structural properties of the (pro)caspase. We show that replacing the residues affects the activity of the procaspase as well as the mature caspase, with D169A and E167A replacements having the largest effects. Replacement of D169 prevents caspase-3 autoactivation, and its cleavage at D175 no longer leads to an active enzyme. In addition, the E173A mutation, when coupled to a second mutation in the procaspase, D175A, may alter the substrate specificity of the procaspase. The mutations affected the active site environment as assessed by changes in fluorescence emission, accessibility to quencher, and cleavage by either trypsin or V8 proteases. High-resolution X-ray crystallographic structures of E167A, D173A, and Y203F caspases show that changes in the active site environment may be due to the increased flexibility of several residues in the N-terminus of the small subunit. Overall, the results show that these residues are important for stabilizing the procaspase active site as well as that of the mature caspase.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17073446}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17073446 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17073446}}
==About this Structure==
==About this Structure==
Line 37: Line 41:
[[Category: Thiol protease]]
[[Category: Thiol protease]]
[[Category: Zymogen]]
[[Category: Zymogen]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:16:07 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:30:19 2008''

Revision as of 10:30, 28 July 2008

Image:2j32.png

Template:STRUCTURE 2j32

THE ROLE OF LOOP BUNDLE HYDROGEN BONDS IN THE MATURATION AND ACTIVITY OF(PRO)CASPASE-3

Template:ABSTRACT PUBMED 17073446

About this Structure

2J32 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Role of loop bundle hydrogen bonds in the maturation and activity of (Pro)caspase-3., Feeney B, Pop C, Swartz P, Mattos C, Clark AC, Biochemistry. 2006 Nov 7;45(44):13249-63. PMID:17073446

Page seeded by OCA on Mon Jul 28 13:30:19 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2j32"
Personal tools