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2j5c

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[[Image:2j5c.gif|left|200px]]
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{{STRUCTURE_2j5c| PDB=2j5c | SCENE= }}
{{STRUCTURE_2j5c| PDB=2j5c | SCENE= }}
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'''RATIONAL CONVERSION OF SUBSTRATE AND PRODUCT SPECIFICITY IN A MONOTERPENE SYNTHASE. STRUCTURAL INSIGHTS INTO THE MOLECULAR BASIS OF RAPID EVOLUTION.'''
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===RATIONAL CONVERSION OF SUBSTRATE AND PRODUCT SPECIFICITY IN A MONOTERPENE SYNTHASE. STRUCTURAL INSIGHTS INTO THE MOLECULAR BASIS OF RAPID EVOLUTION.===
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==Overview==
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Terpene synthases are responsible for the biosynthesis of the complex chemical defense arsenal of plants and microorganisms. How do these enzymes, which all appear to share a common terpene synthase fold, specify the many different products made almost entirely from one of only three substrates? Elucidation of the structure of 1,8-cineole synthase from Salvia fruticosa (Sf-CinS1) combined with analysis of functional and phylogenetic relationships of enzymes within Salvia species identified active-site residues responsible for product specificity. Thus, Sf-CinS1 was successfully converted to a sabinene synthase with a minimum number of rationally predicted substitutions, while identification of the Asn side chain essential for water activation introduced 1,8-cineole and alpha-terpineol activity to Salvia pomifera sabinene synthase. A major contribution to product specificity in Sf-CinS1 appears to come from a local deformation within one of the helices forming the active site. This deformation is observed in all other mono- or sesquiterpene structures available, pointing to a conserved mechanism. Moreover, a single amino acid substitution enlarged the active-site cavity enough to accommodate the larger farnesyl pyrophosphate substrate and led to the efficient synthesis of sesquiterpenes, while alternate single substitutions of this critical amino acid yielded five additional terpene synthases.
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{{ABSTRACT_PUBMED_17557809}}
==About this Structure==
==About this Structure==
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[[Category: Monoterpene]]
[[Category: Monoterpene]]
[[Category: Terpene synthase]]
[[Category: Terpene synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:21:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:46:01 2008''

Revision as of 18:46, 27 July 2008

Image:2j5c.png

Template:STRUCTURE 2j5c

RATIONAL CONVERSION OF SUBSTRATE AND PRODUCT SPECIFICITY IN A MONOTERPENE SYNTHASE. STRUCTURAL INSIGHTS INTO THE MOLECULAR BASIS OF RAPID EVOLUTION.

Template:ABSTRACT PUBMED 17557809

About this Structure

2J5C is a Single protein structure of sequence from Salvia fruticosa. Full crystallographic information is available from OCA.

Reference

Rational conversion of substrate and product specificity in a Salvia monoterpene synthase: structural insights into the evolution of terpene synthase function., Kampranis SC, Ioannidis D, Purvis A, Mahrez W, Ninga E, Katerelos NA, Anssour S, Dunwell JM, Degenhardt J, Makris AM, Goodenough PW, Johnson CB, Plant Cell. 2007 Jun;19(6):1994-2005. Epub 2007 Jun 8. PMID:17557809

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