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| - | [[Image:2jaq.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2jaq| PDB=2jaq | SCENE= }} | | {{STRUCTURE_2jaq| PDB=2jaq | SCENE= }} |
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| - | '''STRUCTURE OF DEOXYADENOSINE KINASE FROM M. MYCOIDES WITH BOUND DCTP'''
| + | ===STRUCTURE OF DEOXYADENOSINE KINASE FROM M. MYCOIDES WITH BOUND DCTP=== |
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| - | ==Overview==
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| - | Deoxyribonucleoside kinases (dNKs) catalyze the transfer of a phosphoryl group from ATP to a deoxyribonucleoside (dN), a key step in DNA precursor synthesis. Recently structural information concerning dNKs has been obtained, but no structure of a bacterial dCK/dGK enzyme is known. Here we report the structure of such an enzyme, represented by deoxyadenosine kinase from Mycoplasma mycoides subsp. mycoides small colony type (Mm-dAK). Superposition of Mm-dAK with its human counterpart's deoxyguanosine kinase (dGK) and deoxycytidine kinase (dCK) reveals that the overall structures are very similar with a few amino acid alterations in the proximity of the active site. To investigate the substrate specificity, Mm-dAK has been crystallized in complex with dATP and dCTP, as well as the products dCMP and dCDP. Both dATP and dCTP bind to the enzyme in a feedback-inhibitory manner with the dN part in the deoxyribonucleoside binding site and the triphosphates in the P-loop. Substrate specificity studies with clinically important nucleoside analogs as well as several phosphate donors were performed. Thus, in this study we combine structural and kinetic data to gain a better understanding of the substrate specificity of the dCK/dGK family of enzymes. The structure of Mm-dAK provides a starting point for making new anti bacterial agents against pathogenic bacteria.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17229440}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17229440 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17229440}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Kinase]] | | [[Category: Kinase]] |
| | [[Category: Transferase]] | | [[Category: Transferase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:37:03 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:45:14 2008'' |
Revision as of 18:45, 28 July 2008
Template:STRUCTURE 2jaq
STRUCTURE OF DEOXYADENOSINE KINASE FROM M. MYCOIDES WITH BOUND DCTP
Template:ABSTRACT PUBMED 17229440
About this Structure
2JAQ is a Single protein structure of sequence from Mycoplasma mycoides subsp. mycoides sc. Full crystallographic information is available from OCA.
Reference
Structure-function analysis of a bacterial deoxyadenosine kinase reveals the basis for substrate specificity., Welin M, Wang L, Eriksson S, Eklund H, J Mol Biol. 2007 Mar 9;366(5):1615-23. Epub 2006 Dec 8. PMID:17229440
Page seeded by OCA on Mon Jul 28 21:45:14 2008
