3psr
From Proteopedia
(New page: 200px<br /> <applet load="3psr" size="450" color="white" frame="true" align="right" spinBox="true" caption="3psr, resolution 2.50Å" /> '''HUMAN PSORIASIN (S1...) |
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- | [[Image:3psr.gif|left|200px]]<br /> | + | [[Image:3psr.gif|left|200px]]<br /><applet load="3psr" size="350" color="white" frame="true" align="right" spinBox="true" |
- | <applet load="3psr" size=" | + | |
caption="3psr, resolution 2.50Å" /> | caption="3psr, resolution 2.50Å" /> | ||
'''HUMAN PSORIASIN (S100A7) CA2+ BOUND FORM (CRYSTAL FORM I)'''<br /> | '''HUMAN PSORIASIN (S100A7) CA2+ BOUND FORM (CRYSTAL FORM I)'''<br /> | ||
==Overview== | ==Overview== | ||
- | The crystal structure of human psoriasin (S100A7) in the native, calcium-bound form has been determined from two crystal forms of the | + | The crystal structure of human psoriasin (S100A7) in the native, calcium-bound form has been determined from two crystal forms of the protein crystallized with and without divalent zinc. The overall structures of the dimeric protein closely resemble the previously determined holmium-substituted structure. The structures also reveal a zinc-binding site of the protein, which is formed by three histidines and an aspartate residue. Together, these residues coordinate the zinc ion in a way similar to the pattern seen in certain metalloproteases and in particular the collagenase family of proteins. Sequence comparison suggests that this zinc site is present in a number of the remaining members of the S100 family. The structure of S100A7 crystallized in the absence of zinc further shows that loss of zinc results in a reorganization of the adjacent empty and distorted EF-hand loop, causing it to resemble a calcium-loaded EF-hand. |
==About this Structure== | ==About this Structure== | ||
- | 3PSR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 3PSR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
- | [[Category: Brodersen, D | + | [[Category: Brodersen, D E.]] |
[[Category: Kjeldgaard, M.]] | [[Category: Kjeldgaard, M.]] | ||
[[Category: Nyborg, J.]] | [[Category: Nyborg, J.]] | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
- | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:11:02 2008'' |
Revision as of 17:11, 21 February 2008
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HUMAN PSORIASIN (S100A7) CA2+ BOUND FORM (CRYSTAL FORM I)
Overview
The crystal structure of human psoriasin (S100A7) in the native, calcium-bound form has been determined from two crystal forms of the protein crystallized with and without divalent zinc. The overall structures of the dimeric protein closely resemble the previously determined holmium-substituted structure. The structures also reveal a zinc-binding site of the protein, which is formed by three histidines and an aspartate residue. Together, these residues coordinate the zinc ion in a way similar to the pattern seen in certain metalloproteases and in particular the collagenase family of proteins. Sequence comparison suggests that this zinc site is present in a number of the remaining members of the S100 family. The structure of S100A7 crystallized in the absence of zinc further shows that loss of zinc results in a reorganization of the adjacent empty and distorted EF-hand loop, causing it to resemble a calcium-loaded EF-hand.
About this Structure
3PSR is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Zinc-binding site of an S100 protein revealed. Two crystal structures of Ca2+-bound human psoriasin (S100A7) in the Zn2+-loaded and Zn2+-free states., Brodersen DE, Nyborg J, Kjeldgaard M, Biochemistry. 1999 Feb 9;38(6):1695-704. PMID:10026247
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