7fab
From Proteopedia
(New page: 200px<br /> <applet load="7fab" size="450" color="white" frame="true" align="right" spinBox="true" caption="7fab, resolution 2.0Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:7fab.gif|left|200px]]<br /> | + | [[Image:7fab.gif|left|200px]]<br /><applet load="7fab" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="7fab" size=" | + | |
caption="7fab, resolution 2.0Å" /> | caption="7fab, resolution 2.0Å" /> | ||
'''CRYSTAL STRUCTURE OF HUMAN IMMUNOGLOBULIN FRAGMENT FAB NEW REFINED AT 2.0 ANGSTROMS RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF HUMAN IMMUNOGLOBULIN FRAGMENT FAB NEW REFINED AT 2.0 ANGSTROMS RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structure of the human immunoglobulin fragment Fab | + | The three-dimensional structure of the human immunoglobulin fragment Fab New (IgG1, lambda) has been refined to a crystallographic R-factor of 16.9% to 2 A resolution. Rms deviations of the final model from ideal geometry are 0.014 A for bond distances and 3.03 degrees for bond angles. Refinement was based on a new X-ray data set including 28,301 reflections with F > 2.5 sigma(F) from 6.0 to 2.0 A resolution. The starting model for the refinement procedure reported here is from the Brookhaven Protein Data Bank entry 3FAB (rev. 1981). Differences between the initial and final models include modified polypeptide-chain folding in the third complementarity-determining region (CDR3) and the third framework region (FR3) of VH and in some exposed loops of CL and CH1. Amino acid sequence changes were determined at a number of positions by inspection of difference electron density maps. The incorporation of amino acid sequence changes results in an improved VH framework model for the "humanization" of monoclonal antibodies. |
==About this Structure== | ==About this Structure== | ||
| - | 7FAB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure | + | 7FAB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entries 3FAB, 2FAB and 1FAB. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FAB OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Poljak, R | + | [[Category: Poljak, R J.]] |
[[Category: Saul, F.]] | [[Category: Saul, F.]] | ||
[[Category: immunoglobulin]] | [[Category: immunoglobulin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:17:05 2008'' |
Revision as of 17:17, 21 February 2008
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CRYSTAL STRUCTURE OF HUMAN IMMUNOGLOBULIN FRAGMENT FAB NEW REFINED AT 2.0 ANGSTROMS RESOLUTION
Overview
The three-dimensional structure of the human immunoglobulin fragment Fab New (IgG1, lambda) has been refined to a crystallographic R-factor of 16.9% to 2 A resolution. Rms deviations of the final model from ideal geometry are 0.014 A for bond distances and 3.03 degrees for bond angles. Refinement was based on a new X-ray data set including 28,301 reflections with F > 2.5 sigma(F) from 6.0 to 2.0 A resolution. The starting model for the refinement procedure reported here is from the Brookhaven Protein Data Bank entry 3FAB (rev. 1981). Differences between the initial and final models include modified polypeptide-chain folding in the third complementarity-determining region (CDR3) and the third framework region (FR3) of VH and in some exposed loops of CL and CH1. Amino acid sequence changes were determined at a number of positions by inspection of difference electron density maps. The incorporation of amino acid sequence changes results in an improved VH framework model for the "humanization" of monoclonal antibodies.
About this Structure
7FAB is a Protein complex structure of sequences from Homo sapiens. This structure supersedes the now removed PDB entries 3FAB, 2FAB and 1FAB. Full crystallographic information is available from OCA.
Reference
Crystal structure of human immunoglobulin fragment Fab New refined at 2.0 A resolution., Saul FA, Poljak RJ, Proteins. 1992 Nov;14(3):363-71. PMID:1438175
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