2jt7

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[[Image:2jt7.jpg|left|200px]]
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{{STRUCTURE_2jt7| PDB=2jt7 | SCENE= }}
{{STRUCTURE_2jt7| PDB=2jt7 | SCENE= }}
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'''NMR solution structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex'''
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===NMR solution structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex===
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==Overview==
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The complex between distamycin A and the parallel DNA quadruplex [d(TGGGGT)]4 has been studied by 1H NMR spectroscopy and isothermal titration calorimetry (ITC). To unambiguously assert that distamycin A interacts with the grooves of the quadruplex [d(TGGGGT)]4, we have analyzed the NMR titration profile of a modified quadruplex, namely [d(TGGMeGGT)]4, and we have applied the recently developed differential frequency-saturation transfer difference (DF-STD) method, for assessing the ligand-DNA binding mode. The three-dimensional structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex has been determined by an in-depth NMR study followed by dynamics and mechanics calculations. All results unequivocally indicate that distamycin molecules interact with [d(TGGGGT)]4 in a 4:1 binding mode, with two antiparallel distamycin dimers that bind simultaneously two opposite grooves of the quadruplex. The affinity between distamycin A and [d(TGGGGT)]4 enhances ( approximately 10-fold) when the ratio of distamycin A to the quadruplex is increased. In this paper we report the first three-dimensional structure of a groove-binder molecule complexed to a DNA quadruplex structure.
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(as it appears on PubMed at http://www.pubmed.gov), where 18052170 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18052170}}
==About this Structure==
==About this Structure==
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2JT7 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JT7 OCA].
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2JT7 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JT7 OCA].
==Reference==
==Reference==
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[[Category: Telomere]]
[[Category: Telomere]]
[[Category: Tggggt]]
[[Category: Tggggt]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:16:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:57:01 2008''

Revision as of 09:57, 27 July 2008

Image:2jt7.png

Template:STRUCTURE 2jt7

NMR solution structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex

Template:ABSTRACT PUBMED 18052170

About this Structure

2JT7 is a Single protein structure. Full experimental information is available from OCA.

Reference

Structural and thermodynamic studies of the interaction of distamycin A with the parallel quadruplex structure [d(TGGGGT)]4., Martino L, Virno A, Pagano B, Virgilio A, Di Micco S, Galeone A, Giancola C, Bifulco G, Mayol L, Randazzo A, J Am Chem Soc. 2007 Dec 26;129(51):16048-56. Epub 2007 Dec 4. PMID:18052170

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