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| - | [[Image:2jtp.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:2jtp.png|left|200px]] |
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| | {{STRUCTURE_2jtp| PDB=2jtp | SCENE= }} | | {{STRUCTURE_2jtp| PDB=2jtp | SCENE= }} |
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| - | '''Solution Structure of the Frameshift-Inducing RNA Stem-Loop in SIV'''
| + | ===Solution Structure of the Frameshift-Inducing RNA Stem-Loop in SIV=== |
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| - | ==Overview==
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| - | Simian immunodeficiency virus (SIV), like its human homologues (HIV-1, HIV-2), requires a -1 translational frameshift event to properly synthesize all of the proteins required for viral replication. The frameshift mechanism is dependent upon a seven-nucleotide slippery sequence and a downstream RNA structure. In SIV, the downstream RNA structure has been proposed to be either a stem-loop or a pseudoknot. Here, we report the functional, structural and thermodynamic characterization of the SIV frameshift site RNA. Translational frameshift assays indicate that a stem-loop structure is sufficient to promote efficient frameshifting in vitro. NMR and thermodynamic studies of SIV RNA constructs of varying length further support the absence of any pseudoknot interaction and indicate the presence of a stable stem-loop structure. We determined the structure of the SIV frameshift-inducing RNA by NMR. The structure reveals a highly ordered 12 nucleotide loop containing a sheared G-A pair, cross-strand adenine stacking, two G-C base-pairs, and a novel CCC triloop turn. The loop structure and its high thermostability preclude pseudoknot formation. Sequence conservation and modeling studies suggest that HIV-2 RNA forms the same structure. We conclude that, like the main sub-groups of HIV-1, SIV and HIV-2 utilize stable stem-loop structures to function as a thermodynamic barrier to translation, thereby inducing ribosomal pausing and frameshifting.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17868691}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17868691 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17868691}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 2JTP is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JTP OCA]. | + | 2JTP is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JTP OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Siv]] | | [[Category: Siv]] |
| | [[Category: Triloop]] | | [[Category: Triloop]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:16:51 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:07:45 2008'' |
Revision as of 09:07, 28 July 2008
Template:STRUCTURE 2jtp
Solution Structure of the Frameshift-Inducing RNA Stem-Loop in SIV
Template:ABSTRACT PUBMED 17868691
About this Structure
2JTP is a Single protein structure. Full experimental information is available from OCA.
Reference
Programmed ribosomal frameshifting in SIV is induced by a highly structured RNA stem-loop., Marcheschi RJ, Staple DW, Butcher SE, J Mol Biol. 2007 Oct 26;373(3):652-63. Epub 2007 Aug 22. PMID:17868691
Page seeded by OCA on Mon Jul 28 12:07:45 2008
