2jvo

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[[Image:2jvo.jpg|left|200px]]
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{{STRUCTURE_2jvo| PDB=2jvo | SCENE= }}
{{STRUCTURE_2jvo| PDB=2jvo | SCENE= }}
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'''Segmental isotope labeling of Npl3'''
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===Segmental isotope labeling of Npl3===
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==Overview==
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The study of multidomain or large proteins in solution by NMR spectroscopy has been made possible in recent years by the development of new spectroscopic methods. However, resonance overlap found in large proteins remains a limiting factor, making resonance assignments and structure determination of large proteins very difficult. In this study, we present an expressed protein ligation protocol that can be used for the segmental isotopic labeling of virtually any multidomain or high molecular mass protein, independent of both the folding state and the solubility of the protein fragments, as well as independent of whether the fragments are interacting. The protocol was applied successfully to two different multidomain proteins containing RNA recognition motifs (RRMs), heterogeneous nuclear ribonucleoprotein L and Npl3p. High yields of segmentally labeled proteins could be obtained, allowing characterization of the interdomain interactions with NMR spectroscopy. We found that the RRMs of heterogeneous nuclear ribonucleoprotein L interact, whereas those of Npl3p are independent. Subsequently, the structures of the two RRMs of Npl3p were determined on the basis of samples in which each RRM was expressed individually. The two Npl3p RRMs adopt the expected beta alpha beta beta alpha beta fold.
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{{ABSTRACT_PUBMED_17936301}}
==About this Structure==
==About this Structure==
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2JVO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JVO OCA].
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2JVO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JVO OCA].
==Reference==
==Reference==
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[[Category: Rna-binding]]
[[Category: Rna-binding]]
[[Category: Rrna processing]]
[[Category: Rrna processing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:50:56 2008''

Revision as of 20:50, 27 July 2008

Image:2jvo.png

Template:STRUCTURE 2jvo

Segmental isotope labeling of Npl3

Template:ABSTRACT PUBMED 17936301

About this Structure

2JVO is a Single protein structure of sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA.

Reference

Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L., Skrisovska L, Allain FH, J Mol Biol. 2008 Jan 4;375(1):151-64. Epub 2007 Sep 16. PMID:17936301

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