2jvr

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[[Image:2jvr.jpg|left|200px]]
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{{Seed}}
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{{STRUCTURE_2jvr| PDB=2jvr | SCENE= }}
{{STRUCTURE_2jvr| PDB=2jvr | SCENE= }}
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'''Segmental Isotope Labeling of Npl3p'''
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===Segmental Isotope Labeling of Npl3p===
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==Overview==
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The study of multidomain or large proteins in solution by NMR spectroscopy has been made possible in recent years by the development of new spectroscopic methods. However, resonance overlap found in large proteins remains a limiting factor, making resonance assignments and structure determination of large proteins very difficult. In this study, we present an expressed protein ligation protocol that can be used for the segmental isotopic labeling of virtually any multidomain or high molecular mass protein, independent of both the folding state and the solubility of the protein fragments, as well as independent of whether the fragments are interacting. The protocol was applied successfully to two different multidomain proteins containing RNA recognition motifs (RRMs), heterogeneous nuclear ribonucleoprotein L and Npl3p. High yields of segmentally labeled proteins could be obtained, allowing characterization of the interdomain interactions with NMR spectroscopy. We found that the RRMs of heterogeneous nuclear ribonucleoprotein L interact, whereas those of Npl3p are independent. Subsequently, the structures of the two RRMs of Npl3p were determined on the basis of samples in which each RRM was expressed individually. The two Npl3p RRMs adopt the expected beta alpha beta beta alpha beta fold.
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(as it appears on PubMed at http://www.pubmed.gov), where 17936301 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17936301}}
==About this Structure==
==About this Structure==
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2JVR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JVR OCA].
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2JVR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JVR OCA].
==Reference==
==Reference==
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[[Category: Rna-binding]]
[[Category: Rna-binding]]
[[Category: Rrna processing]]
[[Category: Rrna processing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:20:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:39:55 2008''

Revision as of 13:39, 28 July 2008

Image:2jvr.png

Template:STRUCTURE 2jvr

Segmental Isotope Labeling of Npl3p

Template:ABSTRACT PUBMED 17936301

About this Structure

2JVR is a Single protein structure of sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA.

Reference

Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L., Skrisovska L, Allain FH, J Mol Biol. 2008 Jan 4;375(1):151-64. Epub 2007 Sep 16. PMID:17936301

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