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2ktx

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{{STRUCTURE_2ktx| PDB=2ktx | SCENE= }}
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'''COMPLETE KALIOTOXIN FROM ANDROCTONUS MAURETANICUS MAURETANICUS, NMR, 18 STRUCTURES'''
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===COMPLETE KALIOTOXIN FROM ANDROCTONUS MAURETANICUS MAURETANICUS, NMR, 18 STRUCTURES===
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==Overview==
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Kaliotoxin (KTX) is a natural peptide blocker of voltage-dependent K+ channels. The 3D structure of a truncated analogue of KTX (Fernandez et al. (1994) Biochemistry 33, 14256-14263) was determined by NMR spectroscopy and showed significant differences from structures established for other related scorpion toxins. A recent publication with the structure of the complete toxin (Aiyar et al. (1995) Neuron 15, 1169-1181) did not confirm these differences. In this communication we report NMR data for KTX at pH 3.0, 5.5 and 7.2 and the 3D structure obtained from data at pH = 5.5. Complete KTX displays a folding similar to that of other toxins with an alpha-helix and a beta-sheet linked by two disulphide bonds. The pKa of His 34 is anomalously low (4.7-5.2 depending on the buffer) owing to its interaction with two Lys residues (including the essential Lys 27), the charged N-terminus and the side chain of Met 29. Charged residues are placed symmetrically with respect to an axis that approximately coincides with one of the principal components of the moment of inertia of the toxin. His 34, which occupies a well-defined position between two conserved Cys, is located on the centre of a layer of charged groups. Positively and negatively charged residues are found at the same position in related toxins. It is suggested that electrostatic effects modulate the distances between positive charges in flexible side chains, contributing to the fine tuning of the selectivity toward different channel subclasses and that the approximate coincidence between the moment of inertia and the charge axis facilitate the approach of the toxin to the channel. The very low pKa of His 34 implies that it will be completely unprotonated at physiological pH.
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{{ABSTRACT_PUBMED_9262650}}
==About this Structure==
==About this Structure==
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2KTX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Androctonus_mauretanicus_mauretanicus Androctonus mauretanicus mauretanicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KTX OCA].
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2KTX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Androctonus_mauretanicus_mauretanicus Androctonus mauretanicus mauretanicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KTX OCA].
==Reference==
==Reference==
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[[Category: Neurotoxin]]
[[Category: Neurotoxin]]
[[Category: Potassium channel inhibitor]]
[[Category: Potassium channel inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:29:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:43:42 2008''

Revision as of 11:43, 27 July 2008

Image:2ktx.png

Template:STRUCTURE 2ktx

COMPLETE KALIOTOXIN FROM ANDROCTONUS MAURETANICUS MAURETANICUS, NMR, 18 STRUCTURES

Template:ABSTRACT PUBMED 9262650

About this Structure

2KTX is a Single protein structure of sequence from Androctonus mauretanicus mauretanicus. Full experimental information is available from OCA.

Reference

3D structure of kaliotoxin: is residue 34 a key for channel selectivity?, Gairi M, Romi R, Fernandez I, Rochat H, Martin-Eauclaire MF, Van Rietschoten J, Pons M, Giralt E, J Pept Sci. 1997 Jul-Aug;3(4):314-9. PMID:9262650

Page seeded by OCA on Sun Jul 27 14:43:42 2008

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