2nlr

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{{STRUCTURE_2nlr| PDB=2nlr | SCENE= }}
{{STRUCTURE_2nlr| PDB=2nlr | SCENE= }}
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'''STREPTOMYCES LIVIDANS ENDOGLUCANASE (EC: 3.2.1.4) COMPLEX WITH MODIFIED GLUCOSE TRIMER'''
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===STREPTOMYCES LIVIDANS ENDOGLUCANASE (EC: 3.2.1.4) COMPLEX WITH MODIFIED GLUCOSE TRIMER===
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==Overview==
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Glycoside hydrolases have been classified into over 66 families on the basis of amino acid sequence. Recently a number of these families have been grouped into "clans" which share a common fold and catalytic mechanism [Henrissat, B., and Bairoch, A. (1996) Biochem. J. 316, 695-696]. Glycoside hydrolase Clan GH-C groups family 11 xylanases and family 12 cellulases, which share the same jellyroll topology, with two predominantly antiparallel beta-sheets forming a long substrate-binding cleft, and act with net retention of anomeric configuration. Here we present the three-dimensional structure of a family 12 endoglucanase, Streptomyces lividans CelB2, in complex with a 2-deoxy-2-fluorocellotrioside. Atomic resolution (1.2 A) data allow clear identification of two distinct species in the crystal. One is the glycosyl-enzyme intermediate, with the mechanism-based inhibitor covalently linked to the nucleophile Glu 120, and the other a complex with the reaction product, 2-deoxy-2-fluoro-beta-D-cellotriose. The active site architecture of the complex provides insight into the double-displacement mechanism of retaining glycoside hydrolases and also sheds light on the basis of the differences in specificity between family 12 cellulases and family 11 xylanases.
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{{ABSTRACT_PUBMED_10200171}}
==About this Structure==
==About this Structure==
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[[Category: Glycosyl hydrolase]]
[[Category: Glycosyl hydrolase]]
[[Category: Glycosyl-enzyme intermediate]]
[[Category: Glycosyl-enzyme intermediate]]
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Revision as of 01:12, 29 July 2008

Image:2nlr.png

Template:STRUCTURE 2nlr

STREPTOMYCES LIVIDANS ENDOGLUCANASE (EC: 3.2.1.4) COMPLEX WITH MODIFIED GLUCOSE TRIMER

Template:ABSTRACT PUBMED 10200171

About this Structure

2NLR is a Single protein structure of sequence from Streptomyces lividans. Full crystallographic information is available from OCA.

Reference

The crystal structure of a 2-fluorocellotriosyl complex of the Streptomyces lividans endoglucanase CelB2 at 1.2 A resolution., Sulzenbacher G, Mackenzie LF, Wilson KS, Withers SG, Dupont C, Davies GJ, Biochemistry. 1999 Apr 13;38(15):4826-33. PMID:10200171

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