2nnw

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{{STRUCTURE_2nnw| PDB=2nnw | SCENE= }}
{{STRUCTURE_2nnw| PDB=2nnw | SCENE= }}
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'''Alternative conformations of Nop56/58-fibrillarin complex and implication for induced-fit assenly of box C/D RNPs'''
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===Alternative conformations of Nop56/58-fibrillarin complex and implication for induced-fit assenly of box C/D RNPs===
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==Overview==
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The Nop56/58-fibrillarin heterocomplex is a core protein complex of the box C/D ribonucleoprotein particles that modify and process ribosomal RNAs. The previous crystal structure of the Archaeoglobus fulgidus complex revealed a symmetric dimer of two Nop56/58-fibrillarin complexes linked by the coiled-coil domains of the Nop56/68 proteins. However, because the A. fulgidus Nop56/58 protein lacks some domains found in most other species, it was thought that the bipartite architecture of the heterocomplex was not likely a general phenomenon. Here we report the crystal structure of the Nop56/58-fibrillarin complex bound with methylation cofactor, S-adenosyl-L-methionine from Pyrococcus furiosus, at 2.7 A. The new complex confirms the generality of the previously observed bipartite arrangement. In addition however, the conformation of Nop56/58 in the new structure differs substantially from that in the earlier structure. The distinct conformations of Nop56/58 suggest potential flexibility in Nop56/58. Computational normal mode analysis supports this view. Importantly, fibrillarin is repositioned within the two complexes. We propose that hinge motion within Nop56/58 has important implications for the possibility of simultaneously positioning two catalytic sites at the two target sites of a bipartite box C/D guide RNA.
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==About this Structure==
==About this Structure==
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[[Category: Box c/d]]
[[Category: Box c/d]]
[[Category: Transferase]]
[[Category: Transferase]]
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Revision as of 13:10, 29 July 2008

Image:2nnw.png

Template:STRUCTURE 2nnw

Alternative conformations of Nop56/58-fibrillarin complex and implication for induced-fit assenly of box C/D RNPs

Template:ABSTRACT PUBMED 17617422

About this Structure

2NNW is a Protein complex structure of sequences from Pyrococcus furiosus. Full crystallographic information is available from OCA.

Reference

Alternative conformations of the archaeal Nop56/58-fibrillarin complex imply flexibility in box C/D RNPs., Oruganti S, Zhang Y, Li H, Robinson H, Terns MP, Terns RM, Yang W, Li H, J Mol Biol. 2007 Aug 31;371(5):1141-50. Epub 2007 Jun 15. PMID:17617422

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