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2ntd

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{{STRUCTURE_2ntd| PDB=2ntd | SCENE= }}
{{STRUCTURE_2ntd| PDB=2ntd | SCENE= }}
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'''Human fibroblast growth factor-1 (140 amino acid form) with Cys117Val/Pro134Cys mutations'''
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===Human fibroblast growth factor-1 (140 amino acid form) with Cys117Val/Pro134Cys mutations===
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==Overview==
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The beta-trefoil protein human fibroblast growth factor-1 (FGF-1) is made up of a six-stranded antiparallel beta-barrel closed off on one end by three beta-hairpins, thus exhibiting a 3-fold axis of structural symmetry. The N and C terminus beta-strands hydrogen bond to each other and their interaction is postulated from both NMR and X-ray structure data to be important in folding and stability. Specific mutations within the adjacent N and C terminus beta-strands of FGF-1 are shown to provide a substantial increase in stability. This increase is largely correlated with an increased folding rate constant, and with a smaller but significant decrease in the unfolding rate constant. A series of stabilizing mutations are subsequently combined and result in a doubling of the DeltaG value of unfolding. When taken in the context of previous studies of stabilizing mutations, the results indicate that although FGF-1 is known for generally poor thermal stability, the beta-trefoil architecture appears capable of substantial thermal stability. Targeting stabilizing mutations within the N and C terminus beta-strand interactions of a beta-barrel architecture may be a generally useful approach to increase protein stability. Such stabilized mutations of FGF-1 are shown to exhibit significant increases in effective mitogenic potency, and may prove useful as "second generation" forms of FGF-1 for application in angiogenic therapy.
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(as it appears on PubMed at http://www.pubmed.gov), where 17570396 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17570396}}
==About this Structure==
==About this Structure==
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[[Category: Dubey, V K.]]
[[Category: Dubey, V K.]]
[[Category: Beta-trefoil]]
[[Category: Beta-trefoil]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:53:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:39:09 2008''

Revision as of 09:39, 29 July 2008

Image:2ntd.png

Template:STRUCTURE 2ntd

Human fibroblast growth factor-1 (140 amino acid form) with Cys117Val/Pro134Cys mutations

Template:ABSTRACT PUBMED 17570396

About this Structure

2NTD is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Spackling the crack: stabilizing human fibroblast growth factor-1 by targeting the N and C terminus beta-strand interactions., Dubey VK, Lee J, Somasundaram T, Blaber S, Blaber M, J Mol Biol. 2007 Aug 3;371(1):256-68. Epub 2007 May 31. PMID:17570396

Page seeded by OCA on Tue Jul 29 12:39:09 2008

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