1d2n

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(New page: 200px<br /> <applet load="1d2n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d2n, resolution 1.75&Aring;" /> '''D2 DOMAIN OF N-ETHY...)
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[[Image:1d2n.gif|left|200px]]<br /><applet load="1d2n" size="350" color="white" frame="true" align="right" spinBox="true"
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<applet load="1d2n" size="450" color="white" frame="true" align="right" spinBox="true"
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caption="1d2n, resolution 1.75&Aring;" />
caption="1d2n, resolution 1.75&Aring;" />
'''D2 DOMAIN OF N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN'''<br />
'''D2 DOMAIN OF N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN'''<br />
==Overview==
==Overview==
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N-ethylmaleimide-sensitive fusion protein (NSF) is a cytosolic ATPase, required for many intracellular vesicle fusion reactions. NSF consists of, an amino-terminal region that interacts with other components of the, vesicle trafficking machinery, followed by two homologous ATP-binding, cassettes, designated D1 and D2, that possess essential ATPase and, hexamerization activities, respectively. The crystal structure of D2 bound, to Mg2+-AMPPNP has been determined at 1.75 A resolution. The structure, consists of a nucleotide-binding and a helical domain, and it is, unexpectedly similar to the first two domains of the clamp-loading subunit, delta' of E. coli DNA polymerase III. The structure suggests several, regions responsible for coupling of ATP hydrolysis to structural changes, in full-length NSF.
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N-ethylmaleimide-sensitive fusion protein (NSF) is a cytosolic ATPase required for many intracellular vesicle fusion reactions. NSF consists of an amino-terminal region that interacts with other components of the vesicle trafficking machinery, followed by two homologous ATP-binding cassettes, designated D1 and D2, that possess essential ATPase and hexamerization activities, respectively. The crystal structure of D2 bound to Mg2+-AMPPNP has been determined at 1.75 A resolution. The structure consists of a nucleotide-binding and a helical domain, and it is unexpectedly similar to the first two domains of the clamp-loading subunit delta' of E. coli DNA polymerase III. The structure suggests several regions responsible for coupling of ATP hydrolysis to structural changes in full-length NSF.
==About this Structure==
==About this Structure==
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1D2N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with MG, ANP and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1D2N with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D2N OCA].
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1D2N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ANP:'>ANP</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1D2N with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D2N OCA].
==Reference==
==Reference==
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[[Category: Cricetulus griseus]]
[[Category: Cricetulus griseus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Lenzen, C.U.]]
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[[Category: Lenzen, C U.]]
[[Category: Steinmann, D.]]
[[Category: Steinmann, D.]]
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[[Category: Weis, W.I.]]
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[[Category: Weis, W I.]]
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[[Category: Whiteheart, S.W.]]
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[[Category: Whiteheart, S W.]]
[[Category: ANP]]
[[Category: ANP]]
[[Category: GOL]]
[[Category: GOL]]
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[[Category: transport]]
[[Category: transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 08:58:40 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:12:17 2008''

Revision as of 10:12, 21 February 2008

Image:1d2n.gif

1d2n, resolution 1.75Å

Drag the structure with the mouse to rotate

D2 DOMAIN OF N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN

Overview

N-ethylmaleimide-sensitive fusion protein (NSF) is a cytosolic ATPase required for many intracellular vesicle fusion reactions. NSF consists of an amino-terminal region that interacts with other components of the vesicle trafficking machinery, followed by two homologous ATP-binding cassettes, designated D1 and D2, that possess essential ATPase and hexamerization activities, respectively. The crystal structure of D2 bound to Mg2+-AMPPNP has been determined at 1.75 A resolution. The structure consists of a nucleotide-binding and a helical domain, and it is unexpectedly similar to the first two domains of the clamp-loading subunit delta' of E. coli DNA polymerase III. The structure suggests several regions responsible for coupling of ATP hydrolysis to structural changes in full-length NSF.

About this Structure

1D2N is a Single protein structure of sequence from Cricetulus griseus with , and as ligands. The following page contains interesting information on the relation of 1D2N with [AAA+ Proteases]. Full crystallographic information is available from OCA.

Reference

Crystal structure of the hexamerization domain of N-ethylmaleimide-sensitive fusion protein., Lenzen CU, Steinmann D, Whiteheart SW, Weis WI, Cell. 1998 Aug 21;94(4):525-36. PMID:9727495

Page seeded by OCA on Thu Feb 21 12:12:17 2008

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