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1d2n
From Proteopedia
(New page: 200px<br /> <applet load="1d2n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d2n, resolution 1.75Å" /> '''D2 DOMAIN OF N-ETHY...) |
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| - | [[Image:1d2n.gif|left|200px]]<br /> | + | [[Image:1d2n.gif|left|200px]]<br /><applet load="1d2n" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1d2n" size=" | + | |
caption="1d2n, resolution 1.75Å" /> | caption="1d2n, resolution 1.75Å" /> | ||
'''D2 DOMAIN OF N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN'''<br /> | '''D2 DOMAIN OF N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | N-ethylmaleimide-sensitive fusion protein (NSF) is a cytosolic ATPase | + | N-ethylmaleimide-sensitive fusion protein (NSF) is a cytosolic ATPase required for many intracellular vesicle fusion reactions. NSF consists of an amino-terminal region that interacts with other components of the vesicle trafficking machinery, followed by two homologous ATP-binding cassettes, designated D1 and D2, that possess essential ATPase and hexamerization activities, respectively. The crystal structure of D2 bound to Mg2+-AMPPNP has been determined at 1.75 A resolution. The structure consists of a nucleotide-binding and a helical domain, and it is unexpectedly similar to the first two domains of the clamp-loading subunit delta' of E. coli DNA polymerase III. The structure suggests several regions responsible for coupling of ATP hydrolysis to structural changes in full-length NSF. |
==About this Structure== | ==About this Structure== | ||
| - | 1D2N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with MG, ANP and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1D2N with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http:// | + | 1D2N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ANP:'>ANP</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1D2N with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D2N OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 14: | ||
[[Category: Cricetulus griseus]] | [[Category: Cricetulus griseus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Lenzen, C | + | [[Category: Lenzen, C U.]] |
[[Category: Steinmann, D.]] | [[Category: Steinmann, D.]] | ||
| - | [[Category: Weis, W | + | [[Category: Weis, W I.]] |
| - | [[Category: Whiteheart, S | + | [[Category: Whiteheart, S W.]] |
[[Category: ANP]] | [[Category: ANP]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
| Line 26: | Line 25: | ||
[[Category: transport]] | [[Category: transport]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:12:17 2008'' |
Revision as of 10:12, 21 February 2008
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D2 DOMAIN OF N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN
Overview
N-ethylmaleimide-sensitive fusion protein (NSF) is a cytosolic ATPase required for many intracellular vesicle fusion reactions. NSF consists of an amino-terminal region that interacts with other components of the vesicle trafficking machinery, followed by two homologous ATP-binding cassettes, designated D1 and D2, that possess essential ATPase and hexamerization activities, respectively. The crystal structure of D2 bound to Mg2+-AMPPNP has been determined at 1.75 A resolution. The structure consists of a nucleotide-binding and a helical domain, and it is unexpectedly similar to the first two domains of the clamp-loading subunit delta' of E. coli DNA polymerase III. The structure suggests several regions responsible for coupling of ATP hydrolysis to structural changes in full-length NSF.
About this Structure
1D2N is a Single protein structure of sequence from Cricetulus griseus with , and as ligands. The following page contains interesting information on the relation of 1D2N with [AAA+ Proteases]. Full crystallographic information is available from OCA.
Reference
Crystal structure of the hexamerization domain of N-ethylmaleimide-sensitive fusion protein., Lenzen CU, Steinmann D, Whiteheart SW, Weis WI, Cell. 1998 Aug 21;94(4):525-36. PMID:9727495
Page seeded by OCA on Thu Feb 21 12:12:17 2008
Categories: AAA+ Proteases | Cricetulus griseus | Single protein | Lenzen, C U. | Steinmann, D. | Weis, W I. | Whiteheart, S W. | ANP | GOL | MG | Atpase | Hexamerization domain | Transport
