1j59
From Proteopedia
(New page: 200px<br /> <applet load="1j59" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j59, resolution 2.50Å" /> '''CATABOLITE GENE ACT...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1j59.gif|left|200px]]<br /> | + | [[Image:1j59.gif|left|200px]]<br /><applet load="1j59" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1j59" size=" | + | |
caption="1j59, resolution 2.50Å" /> | caption="1j59, resolution 2.50Å" /> | ||
'''CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE'''<br /> | '''CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystallographic structure of the CAP-DNA complex at 3.0 A resolution | + | The crystallographic structure of the CAP-DNA complex at 3.0 A resolution has been reported previously. For technical reasons, the reported structure had been determined using a gapped DNA molecule lacking two phosphates important for CAP-DNA interaction. In this work, we report the crystallographic structure of the CAP-DNA complex at 2.5 A resolution using a DNA molecule having all phosphates important for CAP-DNA interaction. The present resolution permits unambiguous identification of amino acid-base and amino acid-phosphate hydrogen bonded contacts in the CAP-DNA complex. In addition, the present resolution permits accurate definition of the kinked DNA conformation in the CAP-DNA complex. |
==About this Structure== | ==About this Structure== | ||
| - | 1J59 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CMP as [http://en.wikipedia.org/wiki/ligand ligand]. This structure | + | 1J59 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CMP:'>CMP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure supersedes the now removed PDB entry 1BER. The following page contains interesting information on the relation of 1J59 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb48_1.html Catabolite Activator Protein]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J59 OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 14: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Berman, H | + | [[Category: Berman, H M.]] |
| - | [[Category: Ebright, R | + | [[Category: Ebright, R H.]] |
| - | [[Category: Ebright, Y | + | [[Category: Ebright, Y W.]] |
[[Category: Gunasekera, A.]] | [[Category: Gunasekera, A.]] | ||
[[Category: Parkinson, G.]] | [[Category: Parkinson, G.]] | ||
| Line 25: | Line 24: | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:19:03 2008'' |
Revision as of 11:19, 21 February 2008
|
CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE
Overview
The crystallographic structure of the CAP-DNA complex at 3.0 A resolution has been reported previously. For technical reasons, the reported structure had been determined using a gapped DNA molecule lacking two phosphates important for CAP-DNA interaction. In this work, we report the crystallographic structure of the CAP-DNA complex at 2.5 A resolution using a DNA molecule having all phosphates important for CAP-DNA interaction. The present resolution permits unambiguous identification of amino acid-base and amino acid-phosphate hydrogen bonded contacts in the CAP-DNA complex. In addition, the present resolution permits accurate definition of the kinked DNA conformation in the CAP-DNA complex.
About this Structure
1J59 is a Single protein structure of sequence from Escherichia coli with as ligand. This structure supersedes the now removed PDB entry 1BER. The following page contains interesting information on the relation of 1J59 with [Catabolite Activator Protein]. Full crystallographic information is available from OCA.
Reference
Structure of the CAP-DNA complex at 2.5 angstroms resolution: a complete picture of the protein-DNA interface., Parkinson G, Wilson C, Gunasekera A, Ebright YW, Ebright RE, Berman HM, J Mol Biol. 1996 Jul 19;260(3):395-408. PMID:8757802
Page seeded by OCA on Thu Feb 21 13:19:03 2008
