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| - | [[Image:2o9f.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2o9f| PDB=2o9f | SCENE= }} | | {{STRUCTURE_2o9f| PDB=2o9f | SCENE= }} |
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| - | '''Crystal Structure of AqpZ mutant L170C'''
| + | ===Crystal Structure of AqpZ mutant L170C=== |
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| - | ==Overview==
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| - | The aquaporin family of channels was defined based on the inhibition of water transport by mercurial compounds. Despite the important role of mercurials, little is known about the structural changes involved upon mercury binding leading to channel inhibition. To elucidate the mechanism we designed a mutant, T183C, of aquaporin Z (AqpZ) patterned after the known mercury-sensitive site of aquaporin 1 (AQP1) and determined the X-ray crystal structures of the unbound and mercury blocked states. Superposition of the two structures shows no conformational rearrangement upon mercury binding. In the blocked structure, there are two mercury sites, one bound to Cys183 and occluding the pore, and a second, also bound to the same cysteine but found buried in an interstitial cavity. To test the mechanism of blockade we designed a different mutant, L170C, to produce a more effective mercury block at the pore site. In a dose-response inhibition study, this mutant was 20 times more sensitive to mercury than wild-type AqpZ and four times more sensitive than T183C. The X-ray structure of L170C shows four mercury atoms at, or near, the pore site defined in the T183C structure and no structural change upon mercury binding. Thus, we elucidate a steric inhibition mechanism for this important class of channels by mercury. | + | The line below this paragraph, {{ABSTRACT_PUBMED_17376483}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17376483 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17376483}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Aquaporin]] | | [[Category: Aquaporin]] |
| | [[Category: Integral membrane protein]] | | [[Category: Integral membrane protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:29:28 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:52:03 2008'' |
Revision as of 09:52, 29 July 2008
Template:STRUCTURE 2o9f
Crystal Structure of AqpZ mutant L170C
Template:ABSTRACT PUBMED 17376483
About this Structure
2O9F is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural basis of aquaporin inhibition by mercury., Savage DF, Stroud RM, J Mol Biol. 2007 May 4;368(3):607-17. Epub 2007 Mar 2. PMID:17376483
Page seeded by OCA on Tue Jul 29 12:52:03 2008
