2o9j

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[[Image:2o9j.gif|left|200px]]
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{{STRUCTURE_2o9j| PDB=2o9j | SCENE= }}
{{STRUCTURE_2o9j| PDB=2o9j | SCENE= }}
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'''Crystal structure of calcium atpase with bound magnesium fluoride and cyclopiazonic acid'''
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===Crystal structure of calcium atpase with bound magnesium fluoride and cyclopiazonic acid===
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==Overview==
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The sarcoplasmic reticulum Ca(2+)-ATPase is essential for calcium reuptake in the muscle contraction-relaxation cycle. Here we present structures of a calcium-free state with bound cyclopiazonic acid (CPA) and magnesium fluoride at 2.65 A resolution and a calcium-free state with bound CPA and ADP at 3.4A resolution. In both structures, CPA occupies the calcium access channel delimited by transmembrane segments M1-M4. Inhibition of Ca(2+)-ATPase is stabilized by a polar pocket that surrounds the tetramic acid of CPA and a hydrophobic platform that cradles the inhibitor. The calcium pump residues involved include Gln(56), Leu(61), Val(62), and Asn(101). We conclude that CPA inhibits the calcium pump by blocking the calcium access channel and immobilizing a subset of transmembrane helices. In the E2(CPA) structure, ADP is bound in a distinct orientation within the nucleotide binding pocket. The adenine ring is sandwiched between Arg(489) of the nucleotide-binding domain and Arg(678) of the phosphorylation domain. This mode of binding conforms to an adenine recognition motif commonly found in ATP-dependent proteins.
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{{ABSTRACT_PUBMED_17259168}}
==About this Structure==
==About this Structure==
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[[Category: Serca]]
[[Category: Serca]]
[[Category: X-ray crystallography]]
[[Category: X-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:29:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:11:40 2008''

Revision as of 09:11, 28 July 2008

Image:2o9j.png

Template:STRUCTURE 2o9j

Crystal structure of calcium atpase with bound magnesium fluoride and cyclopiazonic acid

Template:ABSTRACT PUBMED 17259168

About this Structure

2O9J is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.

Reference

The molecular basis for cyclopiazonic acid inhibition of the sarcoplasmic reticulum calcium pump., Moncoq K, Trieber CA, Young HS, J Biol Chem. 2007 Mar 30;282(13):9748-57. Epub 2007 Jan 26. PMID:17259168

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