1m1j
From Proteopedia
(New page: 200px<br /> <applet load="1m1j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m1j, resolution 2.70Å" /> '''Crystal structure o...) |
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| - | [[Image:1m1j.gif|left|200px]]<br /> | + | [[Image:1m1j.gif|left|200px]]<br /><applet load="1m1j" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1m1j, resolution 2.70Å" /> | caption="1m1j, resolution 2.70Å" /> | ||
'''Crystal structure of native chicken fibrinogen with two different bound ligands'''<br /> | '''Crystal structure of native chicken fibrinogen with two different bound ligands'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of native chicken fibrinogen (320 kDa) complexed | + | The crystal structure of native chicken fibrinogen (320 kDa) complexed with two synthetic peptides has been determined at a resolution of 2.7 A. The structure provides the first atomic-resolution view of the polypeptide chain arrangement in the central domain where the two halves of the molecule are joined, as well as of a putative thrombin-binding site. The amino-terminal segments of the alpha and beta chains, including fibrinopeptides A and B, are not visible in electron density maps, however, and must be highly disordered. The alphaC domain is also very disordered. A residue by residue analysis of the coiled coils with regard to temperature factor shows a strong correlation between mobility and plasmin attack sites. It is concluded that structural flexibility is an inherent feature of fibrinogen that plays a key role in both its conversion to fibrin and its subsequent destruction by plasmin. |
==About this Structure== | ==About this Structure== | ||
| - | 1M1J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with NDG, NAG and CA as [http://en.wikipedia.org/wiki/ligands ligands]. This structure | + | 1M1J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=NDG:'>NDG</scene>, <scene name='pdbligand=NAG:'>NAG</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1JFE. The following page contains interesting information on the relation of 1M1J with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb83_1.html Fibrin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M1J OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Doolittle, R | + | [[Category: Doolittle, R F.]] |
| - | [[Category: Kollman, J | + | [[Category: Kollman, J M.]] |
[[Category: Pandi, L.]] | [[Category: Pandi, L.]] | ||
[[Category: Yang, Z.]] | [[Category: Yang, Z.]] | ||
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[[Category: fibrinogen]] | [[Category: fibrinogen]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:50:30 2008'' |
Revision as of 11:50, 21 February 2008
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Crystal structure of native chicken fibrinogen with two different bound ligands
Overview
The crystal structure of native chicken fibrinogen (320 kDa) complexed with two synthetic peptides has been determined at a resolution of 2.7 A. The structure provides the first atomic-resolution view of the polypeptide chain arrangement in the central domain where the two halves of the molecule are joined, as well as of a putative thrombin-binding site. The amino-terminal segments of the alpha and beta chains, including fibrinopeptides A and B, are not visible in electron density maps, however, and must be highly disordered. The alphaC domain is also very disordered. A residue by residue analysis of the coiled coils with regard to temperature factor shows a strong correlation between mobility and plasmin attack sites. It is concluded that structural flexibility is an inherent feature of fibrinogen that plays a key role in both its conversion to fibrin and its subsequent destruction by plasmin.
About this Structure
1M1J is a Protein complex structure of sequences from Gallus gallus with , and as ligands. This structure supersedes the now removed PDB entry 1JFE. The following page contains interesting information on the relation of 1M1J with [Fibrin]. Full crystallographic information is available from OCA.
Reference
Crystal structure of native chicken fibrinogen at 2.7 A resolution., Yang Z, Kollman JM, Pandi L, Doolittle RF, Biochemistry. 2001 Oct 23;40(42):12515-23. PMID:11601975
Page seeded by OCA on Thu Feb 21 13:50:30 2008
Categories: Fibrin | Gallus gallus | Protein complex | Doolittle, R F. | Kollman, J M. | Pandi, L. | Yang, Z. | CA | NAG | NDG | Coiled coils | Disulfide rings | Fibrinogen
