1n0v
From Proteopedia
(New page: 200px<br /> <applet load="1n0v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n0v, resolution 2.85Å" /> '''Crystal structure o...) |
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| - | [[Image:1n0v.gif|left|200px]]<br /> | + | [[Image:1n0v.gif|left|200px]]<br /><applet load="1n0v" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1n0v" size=" | + | |
caption="1n0v, resolution 2.85Å" /> | caption="1n0v, resolution 2.85Å" /> | ||
'''Crystal structure of elongation factor 2'''<br /> | '''Crystal structure of elongation factor 2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Two crystal structures of yeast translation elongation factor 2 (eEF2) | + | Two crystal structures of yeast translation elongation factor 2 (eEF2) were determined: the apo form at 2.9 A resolution and eEF2 in the presence of the translocation inhibitor sordarin at 2.1 A resolution. The overall conformation of apo eEF2 is similar to that of its prokaryotic homolog elongation factor G (EF-G) in complex with GDP. Upon sordarin binding, the three tRNA-mimicking C-terminal domains undergo substantial conformational changes, while the three N-terminal domains containing the nucleotide-binding site form an almost rigid unit. The conformation of eEF2 in complex with sordarin is entirely different from known conformations observed in crystal structures of EF-G or from cryo-EM studies of EF-G-70S complexes. The domain rearrangements induced by sordarin binding and the highly ordered drug-binding site observed in the eEF2-sordarin structure provide a high-resolution structural basis for the mechanism of sordarin inhibition. The two structures also emphasize the dynamic nature of the ribosomal translocase. |
==About this Structure== | ==About this Structure== | ||
| - | 1N0V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The following page contains interesting information on the relation of 1N0V with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb81_1.html Elongation Factors]]. Full crystallographic information is available from [http:// | + | 1N0V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The following page contains interesting information on the relation of 1N0V with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb81_1.html Elongation Factors]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N0V OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Andersen, G | + | [[Category: Andersen, G R.]] |
[[Category: Carr-Schmid, A.]] | [[Category: Carr-Schmid, A.]] | ||
[[Category: Joergensen, R.]] | [[Category: Joergensen, R.]] | ||
| - | [[Category: Kinzy, T | + | [[Category: Kinzy, T G.]] |
[[Category: Nissen, P.]] | [[Category: Nissen, P.]] | ||
| - | [[Category: Ortiz, P | + | [[Category: Ortiz, P A.]] |
[[Category: g-protein cis-proline]] | [[Category: g-protein cis-proline]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:01 2008'' |
Revision as of 12:01, 21 February 2008
|
Crystal structure of elongation factor 2
Overview
Two crystal structures of yeast translation elongation factor 2 (eEF2) were determined: the apo form at 2.9 A resolution and eEF2 in the presence of the translocation inhibitor sordarin at 2.1 A resolution. The overall conformation of apo eEF2 is similar to that of its prokaryotic homolog elongation factor G (EF-G) in complex with GDP. Upon sordarin binding, the three tRNA-mimicking C-terminal domains undergo substantial conformational changes, while the three N-terminal domains containing the nucleotide-binding site form an almost rigid unit. The conformation of eEF2 in complex with sordarin is entirely different from known conformations observed in crystal structures of EF-G or from cryo-EM studies of EF-G-70S complexes. The domain rearrangements induced by sordarin binding and the highly ordered drug-binding site observed in the eEF2-sordarin structure provide a high-resolution structural basis for the mechanism of sordarin inhibition. The two structures also emphasize the dynamic nature of the ribosomal translocase.
About this Structure
1N0V is a Single protein structure of sequence from Saccharomyces cerevisiae. The following page contains interesting information on the relation of 1N0V with [Elongation Factors]. Full crystallographic information is available from OCA.
Reference
Two crystal structures demonstrate large conformational changes in the eukaryotic ribosomal translocase., Jorgensen R, Ortiz PA, Carr-Schmid A, Nissen P, Kinzy TG, Andersen GR, Nat Struct Biol. 2003 May;10(5):379-85. PMID:12692531
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