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2obg

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{{STRUCTURE_2obg| PDB=2obg | SCENE= }}
{{STRUCTURE_2obg| PDB=2obg | SCENE= }}
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'''Crystal Structure of Monobody MBP-74/Maltose Binding Protein Fusion Complex'''
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===Crystal Structure of Monobody MBP-74/Maltose Binding Protein Fusion Complex===
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==Overview==
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High degrees of sequence and conformation complexity found in natural protein interaction interfaces are generally considered essential for achieving tight and specific interactions. However, it has been demonstrated that specific antibodies can be built by using an interface with a binary code consisting of only Tyr and Ser. This surprising result might be attributed to yet undefined properties of the antibody scaffold that uniquely enhance its capacity for target binding. In this work we tested the generality of the binary-code interface by engineering binding proteins based on a single-domain scaffold. We show that Tyr/Ser binary-code interfaces consisting of only 15-20 positions within a fibronectin type III domain (FN3; 95 residues) are capable of producing specific binding proteins (termed "monobodies") with a low-nanomolar K(d). A 2.35-A x-ray crystal structure of a monobody in complex with its target, maltose-binding protein, and mutation analysis revealed dominant contributions of Tyr residues to binding as well as striking molecular mimicry of a maltose-binding protein substrate, beta-cyclodextrin, by the Tyr/Ser binary interface. This work suggests that an interaction interface with low chemical diversity but with significant conformational diversity is generally sufficient for tight and specific molecular recognition, providing fundamental insights into factors governing protein-protein interactions.
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{{ABSTRACT_PUBMED_17420456}}
==About this Structure==
==About this Structure==
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[[Category: Binding protein]]
[[Category: Binding protein]]
[[Category: Domain swapping]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:33:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:18:15 2008''

Revision as of 18:18, 27 July 2008

Image:2obg.png

Template:STRUCTURE 2obg

Crystal Structure of Monobody MBP-74/Maltose Binding Protein Fusion Complex

Template:ABSTRACT PUBMED 17420456

About this Structure

2OBG is a Single protein structure of sequence from Escherichia coli, synthetic. Full crystallographic information is available from OCA.

Reference

High-affinity single-domain binding proteins with a binary-code interface., Koide A, Gilbreth RN, Esaki K, Tereshko V, Koide S, Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6632-7. Epub 2007 Apr 9. PMID:17420456

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