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1prh
From Proteopedia
(New page: 200px<br /> <applet load="1prh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1prh, resolution 3.5Å" /> '''THE X-RAY CRYSTAL ST...) |
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| - | [[Image:1prh.gif|left|200px]]<br /> | + | [[Image:1prh.gif|left|200px]]<br /><applet load="1prh" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1prh" size=" | + | |
caption="1prh, resolution 3.5Å" /> | caption="1prh, resolution 3.5Å" /> | ||
'''THE X-RAY CRYSTAL STRUCTURE OF THE MEMBRANE PROTEIN PROSTAGLANDIN H2 SYNTHASE-1'''<br /> | '''THE X-RAY CRYSTAL STRUCTURE OF THE MEMBRANE PROTEIN PROSTAGLANDIN H2 SYNTHASE-1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structure of prostaglandin H2 synthase-1, an | + | The three-dimensional structure of prostaglandin H2 synthase-1, an integral membrane protein, has been determined at 3.5 A resolution by X-ray crystallography. This bifunctional enzyme comprises three independent folding units: an epidermal growth factor domain, a membrane-binding motif and an enzymatic domain. Two adjacent but spatially distinct active sites were found for its haem-dependent peroxidase and cyclooxygenase activities. The cyclooxygenase active site is created by a long, hydrophobic channel that is the site of non-steroidal anti-inflammatory drug binding. The conformation of the membrane-binding motif strongly suggests that the enzyme integrates into only one leaflet of the lipid bilayer and is thus a monotopic membrane protein. |
==About this Structure== | ==About this Structure== | ||
| - | 1PRH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1PRH with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb17_1.html Cyclooxygenase]]. Full crystallographic information is available from [http:// | + | 1PRH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] with <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1PRH with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb17_1.html Cyclooxygenase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Ovis aries]] | [[Category: Ovis aries]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Garavito, R | + | [[Category: Garavito, R M.]] |
| - | [[Category: Loll, P | + | [[Category: Loll, P J.]] |
[[Category: Picot, D.]] | [[Category: Picot, D.]] | ||
[[Category: HEM]] | [[Category: HEM]] | ||
| Line 22: | Line 21: | ||
[[Category: peroxidase)]] | [[Category: peroxidase)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:31:47 2008'' |
Revision as of 12:31, 21 February 2008
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THE X-RAY CRYSTAL STRUCTURE OF THE MEMBRANE PROTEIN PROSTAGLANDIN H2 SYNTHASE-1
Overview
The three-dimensional structure of prostaglandin H2 synthase-1, an integral membrane protein, has been determined at 3.5 A resolution by X-ray crystallography. This bifunctional enzyme comprises three independent folding units: an epidermal growth factor domain, a membrane-binding motif and an enzymatic domain. Two adjacent but spatially distinct active sites were found for its haem-dependent peroxidase and cyclooxygenase activities. The cyclooxygenase active site is created by a long, hydrophobic channel that is the site of non-steroidal anti-inflammatory drug binding. The conformation of the membrane-binding motif strongly suggests that the enzyme integrates into only one leaflet of the lipid bilayer and is thus a monotopic membrane protein.
About this Structure
1PRH is a Single protein structure of sequence from Ovis aries with as ligand. The following page contains interesting information on the relation of 1PRH with [Cyclooxygenase]. Full crystallographic information is available from OCA.
Reference
The X-ray crystal structure of the membrane protein prostaglandin H2 synthase-1., Picot D, Loll PJ, Garavito RM, Nature. 1994 Jan 20;367(6460):243-9. PMID:8121489
Page seeded by OCA on Thu Feb 21 14:31:47 2008
