1qys
From Proteopedia
(New page: 200px<br /> <applet load="1qys" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qys, resolution 2.50Å" /> '''Crystal structure o...) |
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| - | [[Image:1qys.gif|left|200px]]<br /> | + | [[Image:1qys.gif|left|200px]]<br /><applet load="1qys" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1qys" size=" | + | |
caption="1qys, resolution 2.50Å" /> | caption="1qys, resolution 2.50Å" /> | ||
'''Crystal structure of Top7: A computationally designed protein with a novel fold'''<br /> | '''Crystal structure of Top7: A computationally designed protein with a novel fold'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A major challenge of computational protein design is the creation of novel | + | A major challenge of computational protein design is the creation of novel proteins with arbitrarily chosen three-dimensional structures. Here, we used a general computational strategy that iterates between sequence design and structure prediction to design a 93-residue alpha/beta protein called Top7 with a novel sequence and topology. Top7 was found experimentally to be folded and extremely stable, and the x-ray crystal structure of Top7 is similar (root mean square deviation equals 1.2 angstroms) to the design model. The ability to design a new protein fold makes possible the exploration of the large regions of the protein universe not yet observed in nature. |
==About this Structure== | ==About this Structure== | ||
| - | 1QYS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Computationally_designed_sequence Computationally designed sequence]. The following page contains interesting information on the relation of 1QYS with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb70_1.html Designer Proteins]]. Full crystallographic information is available from [http:// | + | 1QYS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Computationally_designed_sequence Computationally designed sequence]. The following page contains interesting information on the relation of 1QYS with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb70_1.html Designer Proteins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Baker, D.]] | [[Category: Baker, D.]] | ||
[[Category: Dantas, G.]] | [[Category: Dantas, G.]] | ||
| - | [[Category: Ireton, G | + | [[Category: Ireton, G C.]] |
[[Category: Kuhlman, B.]] | [[Category: Kuhlman, B.]] | ||
| - | [[Category: Stoddard, B | + | [[Category: Stoddard, B L.]] |
[[Category: Varani, G.]] | [[Category: Varani, G.]] | ||
[[Category: alpha-beta]] | [[Category: alpha-beta]] | ||
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[[Category: novel fold]] | [[Category: novel fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:45:08 2008'' |
Revision as of 12:45, 21 February 2008
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Crystal structure of Top7: A computationally designed protein with a novel fold
Overview
A major challenge of computational protein design is the creation of novel proteins with arbitrarily chosen three-dimensional structures. Here, we used a general computational strategy that iterates between sequence design and structure prediction to design a 93-residue alpha/beta protein called Top7 with a novel sequence and topology. Top7 was found experimentally to be folded and extremely stable, and the x-ray crystal structure of Top7 is similar (root mean square deviation equals 1.2 angstroms) to the design model. The ability to design a new protein fold makes possible the exploration of the large regions of the protein universe not yet observed in nature.
About this Structure
1QYS is a Protein complex structure of sequences from Computationally designed sequence. The following page contains interesting information on the relation of 1QYS with [Designer Proteins]. Full crystallographic information is available from OCA.
Reference
Design of a novel globular protein fold with atomic-level accuracy., Kuhlman B, Dantas G, Ireton GC, Varani G, Stoddard BL, Baker D, Science. 2003 Nov 21;302(5649):1364-8. PMID:14631033
Page seeded by OCA on Thu Feb 21 14:45:08 2008
