1rlc
From Proteopedia
(New page: 200px<br /> <applet load="1rlc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rlc, resolution 2.7Å" /> '''CRYSTAL STRUCTURE OF...) |
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caption="1rlc, resolution 2.7Å" /> | caption="1rlc, resolution 2.7Å" /> | ||
'''CRYSTAL STRUCTURE OF THE UNACTIVATED RIBULOSE 1, 5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE COMPLEXED WITH A TRANSITION STATE ANALOG, 2-CARBOXY-D-ARABINITOL 1,5-BISPHOSPHATE'''<br /> | '''CRYSTAL STRUCTURE OF THE UNACTIVATED RIBULOSE 1, 5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE COMPLEXED WITH A TRANSITION STATE ANALOG, 2-CARBOXY-D-ARABINITOL 1,5-BISPHOSPHATE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1RLC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum] with CAP as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1RLC with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb11_1.html Rubisco]]. Active as [http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39] Full crystallographic information is available from [http:// | + | 1RLC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum] with <scene name='pdbligand=CAP:'>CAP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1RLC with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb11_1.html Rubisco]]. Active as [http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RLC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: lyase(carbon-carbon)]] | [[Category: lyase(carbon-carbon)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:48:58 2008'' |
Revision as of 14:48, 15 February 2008
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CRYSTAL STRUCTURE OF THE UNACTIVATED RIBULOSE 1, 5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE COMPLEXED WITH A TRANSITION STATE ANALOG, 2-CARBOXY-D-ARABINITOL 1,5-BISPHOSPHATE
Overview
The crystal structure of unactivated ribulose 1,5-bisphosphate, carboxylase/oxygenase from Nicotiana tabacum complexed with a transition, state analog, 2-carboxy-D-arabinitol 1,5-bisphosphate, was determined to, 2.7 A resolution by X-ray crystallography. The transition state analog, binds at the active site in an extended conformation. As compared to the, binding of the same analog in the activated enzyme, the analog binds in a, reverse orientation. The active site Lys 201 is within hydrogen bonding, distance of the carboxyl oxygen of the analog. Loop 6 (residues 330-339), remains open and flexible upon binding of the analog in the unactivated, enzyme, in contrast to the closed and ordered loop 6 in the activated, enzyme complex. The transition state analog is exposed to solvent due to, the open conformation of loop 6.
About this Structure
1RLC is a Protein complex structure of sequences from Nicotiana tabacum with as ligand. The following page contains interesting information on the relation of 1RLC with [Rubisco]. Active as Ribulose-bisphosphate carboxylase, with EC number 4.1.1.39 Full crystallographic information is available from OCA.
Reference
Crystal structure of the unactivated ribulose 1,5-bisphosphate carboxylase/oxygenase complexed with a transition state analog, 2-carboxy-D-arabinitol 1,5-bisphosphate., Zhang KY, Cascio D, Eisenberg D, Protein Sci. 1994 Jan;3(1):64-9. PMID:8142899
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