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| | {{STRUCTURE_2ofd| PDB=2ofd | SCENE= }} | | {{STRUCTURE_2ofd| PDB=2ofd | SCENE= }} |
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| - | '''The Crystal Structure of Sclerotium rolfsii lectin in complex with N-acetyl-D-galactosamine'''
| + | ===The Crystal Structure of Sclerotium rolfsii lectin in complex with N-acetyl-D-galactosamine=== |
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| - | ==Overview==
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| - | The crystal structure of a novel fungal lectin from Sclerotium rolfsii (SRL) in its free form and in complex with N-acetyl-d-galactosamine (GalNAc) and N-acetyl- d -glucosamine (GlcNAc) has been determined at 1.1 A, 2.0 A, and 1.7 A resolution, respectively. The protein structure is composed of two beta-sheets, which consist of four and six beta-strands, connected by two alpha-helices. Sequence and structural comparisons reveal that SRL is the third member of a newly identified family of fungal lectins, which includes lectins from Agaricus bisporus and Xerocomus chrysenteron that share a high degree of structural similarity and carbohydrate specificity. The data for the free SRL are the highest resolution data for any protein of this family. The crystal structures of the SRL in complex with two carbohydrates, GalNAc and GlcNAc, which differ only in the configuration of a single epimeric hydroxyl group, provide the structural basis for its carbohydrate specificity. SRL has two distinct carbohydrate-binding sites, a primary and a secondary. GalNAc binds at the primary site, whereas GlcNAc binds only at the secondary site. Thus, SRL has the ability to recognize and probably bind at the same time two different carbohydrate structures. Structural comparison to Agaricus bisporus lectin-carbohydrate complexes reveals that the primary site is also able to bind the Thomsen-Friedenreich antigen (Galbeta1-->3GalNAc-alpha- glycan structures) whereas the secondary site cannot. The features of the molecular recognition at the two sites are described in detail.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17391699}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17391699 is the PubMed ID number. |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Dual specificity]] | | [[Category: Dual specificity]] |
| | [[Category: Lectin]] | | [[Category: Lectin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:47:38 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:35:55 2008'' |
Revision as of 17:36, 28 July 2008
Template:STRUCTURE 2ofd
The Crystal Structure of Sclerotium rolfsii lectin in complex with N-acetyl-D-galactosamine
Template:ABSTRACT PUBMED 17391699
About this Structure
Full crystallographic information is available from OCA.
Reference
Structural basis for the carbohydrate recognition of the Sclerotium rolfsii lectin., Leonidas DD, Swamy BM, Hatzopoulos GN, Gonchigar SJ, Chachadi VB, Inamdar SR, Zographos SE, Oikonomakos NG, J Mol Biol. 2007 May 11;368(4):1145-61. Epub 2007 Mar 7. PMID:17391699
Page seeded by OCA on Mon Jul 28 20:35:55 2008
