1h8d
From Proteopedia
(New page: 200px<br /> <applet load="1h8d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h8d, resolution 1.4Å" /> '''X-RAY STRUCTURE OF T...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1H8D is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis]] and [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with PHW as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H8D OCA]]. | + | 1H8D is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis]] and [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with PHW as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Thrombin Thrombin]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H8D OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| + | [[Category: Thrombin]] | ||
[[Category: Deadman, J.]] | [[Category: Deadman, J.]] | ||
[[Category: Dodson, G.G.]] | [[Category: Dodson, G.G.]] | ||
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[[Category: serine protease]] | [[Category: serine protease]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:23:33 2007'' |
Revision as of 10:18, 30 October 2007
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X-RAY STRUCTURE OF THE HUMAN ALPHA-THROMBIN COMPLEX WITH A TRIPEPTIDE PHOSPHONATE INHIBITOR.
Overview
X-ray crystallographic studies of human alpha-thrombin with a novel, synthetic inhibitor, an acyl (alpha-aminoalkyl)phosphonate, reveal the, existence of a pentacovalent phosphorus intermediate state. Crystal, structures of the complex of alpha-thrombin with the phosphonate compound, were determined independently using crystals of different ages. The first, structure, solved from a crystal less than seven days old, showed a, pentacoordinated phosphorus moiety. The second structure, determined from, a crystal that was 12 weeks old, showed a tetracoordinated phosphorus, moiety. In the first structure, a water molecule, made nucleophilic by, coordination to His57 of alpha-thrombin, is bonded to the pentacoordinated, phosphorus atom. Its position is approximately equivalent to that occupied, ... [(full description)]
About this Structure
1H8D is a [Protein complex] structure of sequences from [Hirudo medicinalis] and [Homo sapiens] with PHW as [ligand]. Active as [Thrombin], with EC number [3.4.21.5]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Inhibition of human alpha-thrombin by a phosphonate tripeptide proceeds via a metastable pentacoordinated phosphorus intermediate., Skordalakes E, Dodson GG, Green DS, Goodwin CA, Scully MF, Hudson HR, Kakkar VV, Deadman JJ, J Mol Biol. 2001 Aug 17;311(3):549-55. PMID:11493008
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