1zaa
From Proteopedia
(New page: 200px<br /> <applet load="1zaa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zaa, resolution 2.100Å" /> '''ZINC FINGER-DNA RE...) |
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| - | [[Image:1zaa. | + | [[Image:1zaa.jpg|left|200px]]<br /><applet load="1zaa" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1zaa, resolution 2.100Å" /> | caption="1zaa, resolution 2.100Å" /> | ||
'''ZINC FINGER-DNA RECOGNITION: CRYSTAL STRUCTURE OF A ZIF268-DNA COMPLEX AT 2.1 ANGSTROMS'''<br /> | '''ZINC FINGER-DNA RECOGNITION: CRYSTAL STRUCTURE OF A ZIF268-DNA COMPLEX AT 2.1 ANGSTROMS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZAA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1ZAA with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb87_1.html Zinc Fingers]]. Full crystallographic information is available from [http:// | + | 1ZAA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1ZAA with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb87_1.html Zinc Fingers]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZAA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:13:09 2008'' |
Revision as of 15:13, 15 February 2008
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ZINC FINGER-DNA RECOGNITION: CRYSTAL STRUCTURE OF A ZIF268-DNA COMPLEX AT 2.1 ANGSTROMS
Overview
The zinc finger DNA-binding motif occurs in many proteins that regulate, eukaryotic gene expression. The crystal structure of a complex containing, the three zinc fingers from Zif268 (a mouse immediate early protein) and a, consensus DNA-binding site has been determined at 2.1 angstroms resolution, and refined to a crystallographic R factor of 18.2 percent. In this, complex, the zinc fingers bind in the major groove of B-DNA and wrap part, way around the double helix. Each finger has a similar relation to the DNA, and makes its primary contacts in a three-base pair subsite. Residues from, the amino-terminal portion of an alpha helix contact the bases, and most, of the contracts are made with the guanine-rich strand of the DNA. This, structure provides a framework for understanding how zinc fingers, recognize DNA and suggests that this motif may provide a useful basis for, the design of novel DNA-binding proteins.
About this Structure
1ZAA is a Single protein structure of sequence from Mus musculus with as ligand. The following page contains interesting information on the relation of 1ZAA with [Zinc Fingers]. Full crystallographic information is available from OCA.
Reference
Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A., Pavletich NP, Pabo CO, Science. 1991 May 10;252(5007):809-17. PMID:2028256
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