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2oml

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[[Image:2oml.gif|left|200px]]
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{{STRUCTURE_2oml| PDB=2oml | SCENE= }}
{{STRUCTURE_2oml| PDB=2oml | SCENE= }}
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'''crystal structure of E. coli pseudouridine synthase RluE'''
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===crystal structure of E. coli pseudouridine synthase RluE===
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==Overview==
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Pseudouridine synthase RluE modifies U2457 in a stem of 23 S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome. We determined the crystal structures of the C-terminal, catalytic domain of E. coli RluE at 1.2 A resolution and of full-length RluE at 1.6 A resolution. The crystals of the full-length enzyme contain two molecules in the asymmetric unit and in both molecules the N-terminal domain is disordered. The protein has an active site cleft, conserved in all other pseudouridine synthases, that contains invariant Asp and Tyr residues implicated in catalysis. An electropositive surface patch that covers the active site cleft is just wide enough to accommodate an RNA stem. The RNA substrate stem can be docked to this surface such that the catalytic Asp is adjacent to the target base, and a conserved Arg is positioned to help flip the target base out of the stem into the enzyme active site. A flexible RluE specific loop lies close to the conserved region of the stem in the model, and may contribute to substrate specificity. The stem alone is not a good RluE substrate, suggesting RluE makes additional interactions with other regions in the ribosome.
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(as it appears on PubMed at http://www.pubmed.gov), where 17320904 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17320904}}
==About this Structure==
==About this Structure==
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[[Category: Bifurcated beta sheet]]
[[Category: Bifurcated beta sheet]]
[[Category: Thrombin-cleaved]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:13:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:58:11 2008''

Revision as of 20:58, 28 July 2008

Image:2oml.png

Template:STRUCTURE 2oml

crystal structure of E. coli pseudouridine synthase RluE

Template:ABSTRACT PUBMED 17320904

About this Structure

2OML is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The crystal structure of E. coli rRNA pseudouridine synthase RluE., Pan H, Ho JD, Stroud RM, Finer-Moore J, J Mol Biol. 2007 Apr 13;367(5):1459-70. Epub 2007 Feb 7. PMID:17320904

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