2onj
From Proteopedia
(New page: 200px<br /> <applet load="2onj" size="450" color="white" frame="true" align="right" spinBox="true" caption="2onj, resolution 3.400Å" /> '''Structure of the m...) |
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| - | [[Image:2onj.gif|left|200px]]<br /> | + | [[Image:2onj.gif|left|200px]]<br /><applet load="2onj" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2onj" size=" | + | |
caption="2onj, resolution 3.400Å" /> | caption="2onj, resolution 3.400Å" /> | ||
'''Structure of the multidrug ABC transporter Sav1866 from S. aureus in complex with AMP-PNP'''<br /> | '''Structure of the multidrug ABC transporter Sav1866 from S. aureus in complex with AMP-PNP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Staphylococcus aureus Sav1866 is a bacterial homolog of the human ABC | + | Staphylococcus aureus Sav1866 is a bacterial homolog of the human ABC transporter Mdr1 that causes multidrug resistance in cancer cells. We report the crystal structure of Sav1866 in complex with adenosine-5'-(beta,gamma-imido)triphosphate (AMP-PNP) at 3.4A resolution and compare it with the previously determined structure of Sav1866 with bound ADP. Besides differences in the ATP-binding sites, no significant conformational changes were observed. The results confirm that the ATP-bound state of multidrug ABC transporters is coupled to an outward-facing conformation of the transmembrane domains. |
==About this Structure== | ==About this Structure== | ||
| - | 2ONJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with NA and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2ONJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=ANP:'>ANP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ONJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
| - | [[Category: Dawson, R | + | [[Category: Dawson, R J.P.]] |
| - | [[Category: Locher, K | + | [[Category: Locher, K P.]] |
[[Category: ANP]] | [[Category: ANP]] | ||
[[Category: NA]] | [[Category: NA]] | ||
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[[Category: transport protein]] | [[Category: transport protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:20:26 2008'' |
Revision as of 16:20, 21 February 2008
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Structure of the multidrug ABC transporter Sav1866 from S. aureus in complex with AMP-PNP
Overview
Staphylococcus aureus Sav1866 is a bacterial homolog of the human ABC transporter Mdr1 that causes multidrug resistance in cancer cells. We report the crystal structure of Sav1866 in complex with adenosine-5'-(beta,gamma-imido)triphosphate (AMP-PNP) at 3.4A resolution and compare it with the previously determined structure of Sav1866 with bound ADP. Besides differences in the ATP-binding sites, no significant conformational changes were observed. The results confirm that the ATP-bound state of multidrug ABC transporters is coupled to an outward-facing conformation of the transmembrane domains.
About this Structure
2ONJ is a Single protein structure of sequence from Staphylococcus aureus with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of the multidrug ABC transporter Sav1866 from Staphylococcus aureus in complex with AMP-PNP., Dawson RJ, Locher KP, FEBS Lett. 2007 Mar 6;581(5):935-8. Epub 2007 Feb 7. PMID:17303126
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