2onb

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[[Image:2onb.gif|left|200px]]
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{{STRUCTURE_2onb| PDB=2onb | SCENE= }}
{{STRUCTURE_2onb| PDB=2onb | SCENE= }}
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'''Human Thymidylate Synthase at low salt conditions with PDPA bound'''
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===Human Thymidylate Synthase at low salt conditions with PDPA bound===
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==Overview==
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Thymidylate synthase (TS) is a target in the chemotherapy of colorectal cancer and some other neoplasms. It catalyzes the transfer of a methyl group from methylenetetrahydrofolate to dUMP to form dTMP. On the basis of structural considerations, we have introduced 1,3-propanediphosphonic acid (PDPA) as an allosteric inhibitor of human TS (hTS); it is proposed that PDPA acts by stabilizing an inactive conformer of loop 181-197. Kinetic studies showed that PDPA is a mixed (noncompetitive) inhibitor versus dUMP. In contrast, versus methylenetrahydrofolate at concentrations lower than 0.25 microM, PDPA is an uncompetitive inhibitor, while at PDPA concentrations higher than 1 microM the inhibiton is noncompetive, as expected. At the concentrations corresponding to uncompetitive inhibition, PDPA shows positive cooperativity with an antifolate inhibitor, ZD9331, which binds to the active conformer. PDPA binding leads to the formation of hTS tetramers, but not higher oligomers. These data are consistent with a model in which hTS exists preferably as an asymmetric dimer with one subunit in the active conformation of loop 181-197 and the other in the inactive conformation.
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(as it appears on PubMed at http://www.pubmed.gov), where 17297914 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17297914}}
==About this Structure==
==About this Structure==
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[[Category: Heteroinhibition]]
[[Category: Heteroinhibition]]
[[Category: Zd9331]]
[[Category: Zd9331]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:15:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:03:39 2008''

Revision as of 12:03, 29 July 2008

Image:2onb.png

Template:STRUCTURE 2onb

Human Thymidylate Synthase at low salt conditions with PDPA bound

Template:ABSTRACT PUBMED 17297914

About this Structure

2ONB is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Cooperative inhibition of human thymidylate synthase by mixtures of active site binding and allosteric inhibitors., Lovelace LL, Gibson LM, Lebioda L, Biochemistry. 2007 Mar 13;46(10):2823-30. Epub 2007 Feb 13. PMID:17297914

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