This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3sdp
From Proteopedia
(New page: 200px<br /> <applet load="3sdp" size="450" color="white" frame="true" align="right" spinBox="true" caption="3sdp, resolution 2.1Å" /> '''THE 2.1 ANGSTROMS RE...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:3sdp. | + | [[Image:3sdp.jpg|left|200px]]<br /><applet load="3sdp" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="3sdp" size=" | + | |
caption="3sdp, resolution 2.1Å" /> | caption="3sdp, resolution 2.1Å" /> | ||
'''THE 2.1 ANGSTROMS RESOLUTION STRUCTURE OF IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS'''<br /> | '''THE 2.1 ANGSTROMS RESOLUTION STRUCTURE OF IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 3SDP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with FE as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 3SDP with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb94_1.html Superoxide Dismutase]]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Full crystallographic information is available from [http:// | + | 3SDP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with <scene name='pdbligand=FE:'>FE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 3SDP with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb94_1.html Superoxide Dismutase]]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDP OCA]. |
==Reference== | ==Reference== | ||
| Line 22: | Line 21: | ||
[[Category: oxidoreductase (superoxide acceptor)]] | [[Category: oxidoreductase (superoxide acceptor)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:44:16 2008'' |
Revision as of 15:44, 15 February 2008
|
THE 2.1 ANGSTROMS RESOLUTION STRUCTURE OF IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS
Overview
The 2.1-A resolution crystal structure of native uncomplexed iron, superoxide dismutase (EC 1.15.1.1) from Pseudomonas ovalis was solved and, refined to a final R factor of 24%. The dimeric structure contains one, catalytic iron center per monomer with an asymmetric trigonal-bipyramidal, coordination of protein ligands to the metal. Each monomer contains two, domains, with the trigonal ligands (histidines 74 and 160; aspartate 156), contributed by the large domain and stabilized by an extended, hydrogen-bonded network, including residues from opposing monomers. The, axial ligand (histidine 26) is found on the small domain and does not, participate extensively in the stabilizing H-bond network. The open axial, coordination position of the iron is devoid of bound water molecules or, anions. The metal is located 0.5 A out of the plane of the trigonal, ligands toward histidine 26, providing a slightly skewed coordination away, from the iron binding site. The molecule contains a glutamine residue in, the active site which is conserved between all iron enzymes sequenced to, data but which is conserved among all manganese SODs at a separate, position in the sequence. This residue shows the same structural, interactions in both cases, implying that iron and manganese SODs are, second-site revertants of one another.
About this Structure
3SDP is a Single protein structure of sequence from Pseudomonas putida with as ligand. The following page contains interesting information on the relation of 3SDP with [Superoxide Dismutase]. Active as Superoxide dismutase, with EC number 1.15.1.1 Full crystallographic information is available from OCA.
Reference
The 2.1-A resolution structure of iron superoxide dismutase from Pseudomonas ovalis., Stoddard BL, Howell PL, Ringe D, Petsko GA, Biochemistry. 1990 Sep 25;29(38):8885-93. PMID:2271564
Page seeded by OCA on Fri Feb 15 17:44:16 2008
