This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2oqs

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2oqs.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2oqs.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2oqs| PDB=2oqs | SCENE= }}
{{STRUCTURE_2oqs| PDB=2oqs | SCENE= }}
-
'''Structure of the hDLG/SAP97 PDZ2 in complex with HPV-18 papillomavirus E6 peptide'''
+
===Structure of the hDLG/SAP97 PDZ2 in complex with HPV-18 papillomavirus E6 peptide===
-
==Overview==
+
<!--
-
The E6 protein from high-risk types of human papillomavirus (HPV) binds PDZ-domain containing proteins and targets them for degradation. We used isothermal titration calorimetry to measure the interaction of a peptide from the C-terminus of HPV-18 E6 to the second PDZ domain (PDZ2) from the human homologue of the Drosophila discs large tumor suppressor protein (hDlg). Isothermal titration calorimetry experiments with a series of peptides showed that HPV-18 E6 bound hDlg PDZ2 about 5-fold stronger than HPV-16 E6, that the contribution of Arg154 to binding was about 1 kcal/mol, and that the binding was disabled by phosphorylation at Thr156. We then used NMR to determine the solution structure of the complex of PDZ2 bound to the HPV-18 E6 peptide. The resultant structures were of high quality and had backbone root-mean-square deviations of less than 0.5 A. The structure shows a novel mode of interaction in which six residues of the HPV-18 E6 peptide are contacted by the PDZ2 domain, in contrast to the typical four residues used by class I PDZ domains. Molecular dynamics simulations supported a model in which the C- and N-terminal ends of the peptide had different mobilities within the complex. Comparison of the NMR complex structure to previously determined X-ray structures of PDZ2 by itself and bound to different peptides allows a description of conformational changes required for PDZ2 to bind to HPV-18 E6.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17713926}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17713926 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17713926}}
==About this Structure==
==About this Structure==
-
2OQS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQS OCA].
+
2OQS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQS OCA].
==Reference==
==Reference==
Line 30: Line 34:
[[Category: Peptide-binding protein]]
[[Category: Peptide-binding protein]]
[[Category: Protein-peptide complex]]
[[Category: Protein-peptide complex]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:28:43 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:54:49 2008''

Revision as of 09:55, 28 July 2008

Image:2oqs.png

Template:STRUCTURE 2oqs

Structure of the hDLG/SAP97 PDZ2 in complex with HPV-18 papillomavirus E6 peptide

Template:ABSTRACT PUBMED 17713926

About this Structure

2OQS is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Solution structure of the hDlg/SAP97 PDZ2 domain and its mechanism of interaction with HPV-18 papillomavirus E6 protein., Liu Y, Henry GD, Hegde RS, Baleja JD, Biochemistry. 2007 Sep 25;46(38):10864-74. Epub 2007 Aug 22. PMID:17713926

Page seeded by OCA on Mon Jul 28 12:54:49 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2oqs"
Personal tools