8cat
From Proteopedia
(New page: 200px<br /> <applet load="8cat" size="450" color="white" frame="true" align="right" spinBox="true" caption="8cat, resolution 2.5Å" /> '''THE NADPH BINDING SI...) |
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| - | [[Image:8cat.gif|left|200px]]<br /> | + | [[Image:8cat.gif|left|200px]]<br /><applet load="8cat" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="8cat" size=" | + | |
caption="8cat, resolution 2.5Å" /> | caption="8cat, resolution 2.5Å" /> | ||
'''THE NADPH BINDING SITE ON BEEF LIVER CATALASE'''<br /> | '''THE NADPH BINDING SITE ON BEEF LIVER CATALASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Beef liver and human erythrocyte catalases (EC 1.11.1.6) bind NADP | + | Beef liver and human erythrocyte catalases (EC 1.11.1.6) bind NADP tenaciously [Kirkman, H. N. & Gaetani, G. F. (1984) Proc. Natl. Acad. Sci. USA 81, 4343-4348]. The position of NADP on beef liver catalase corresponds to the carboxyl-terminal polypeptide hinge in Penicillium vitale fungal catalase, which connects the common catalase structure to the additional flavodoxin-like domain. In contrast to nearly all other known structures of protein-bound NADP, NAD, and FAD, the NADP molecule of beef liver catalase is folded into a right-handed helix and bound, in part, in the vicinity of the carboxyl end of two alpha-helices. A water molecule (W7) occupies a pseudosubstrate site close to the C4 position of the nicotinamide and is hydrogen bonded to His-304. Although the NADP and heme groups approach each other to within 13.7 A, there is no direct interaction. The function of the NADP remains a mystery. |
==About this Structure== | ==About this Structure== | ||
| - | 8CAT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with HEM and NDP as [http://en.wikipedia.org/wiki/ligands ligands]. This structure | + | 8CAT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=NDP:'>NDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entries 3CAT and 1CAT. The following page contains interesting information on the relation of 8CAT with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb57_1.html Catalase]]. Active as [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CAT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Catalase]] | [[Category: Catalase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: /III, T | + | [[Category: /III, T J.Reid.]] |
[[Category: Fita, I.]] | [[Category: Fita, I.]] | ||
| - | [[Category: Murthy, M | + | [[Category: Murthy, M R.N.]] |
| - | [[Category: Rossmann, M | + | [[Category: Rossmann, M G.]] |
[[Category: Sicignano, A.]] | [[Category: Sicignano, A.]] | ||
[[Category: Tanaka, N.]] | [[Category: Tanaka, N.]] | ||
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[[Category: oxidoreductase (h2o2 acceptor)]] | [[Category: oxidoreductase (h2o2 acceptor)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:17:50 2008'' |
Revision as of 17:17, 21 February 2008
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THE NADPH BINDING SITE ON BEEF LIVER CATALASE
Overview
Beef liver and human erythrocyte catalases (EC 1.11.1.6) bind NADP tenaciously [Kirkman, H. N. & Gaetani, G. F. (1984) Proc. Natl. Acad. Sci. USA 81, 4343-4348]. The position of NADP on beef liver catalase corresponds to the carboxyl-terminal polypeptide hinge in Penicillium vitale fungal catalase, which connects the common catalase structure to the additional flavodoxin-like domain. In contrast to nearly all other known structures of protein-bound NADP, NAD, and FAD, the NADP molecule of beef liver catalase is folded into a right-handed helix and bound, in part, in the vicinity of the carboxyl end of two alpha-helices. A water molecule (W7) occupies a pseudosubstrate site close to the C4 position of the nicotinamide and is hydrogen bonded to His-304. Although the NADP and heme groups approach each other to within 13.7 A, there is no direct interaction. The function of the NADP remains a mystery.
About this Structure
8CAT is a Single protein structure of sequence from Bos taurus with and as ligands. This structure supersedes the now removed PDB entries 3CAT and 1CAT. The following page contains interesting information on the relation of 8CAT with [Catalase]. Active as Catalase, with EC number 1.11.1.6 Full crystallographic information is available from OCA.
Reference
The NADPH binding site on beef liver catalase., Fita I, Rossmann MG, Proc Natl Acad Sci U S A. 1985 Mar;82(6):1604-8. PMID:3856839
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