2oww

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[[Image:2oww.jpg|left|200px]]
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{{Seed}}
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{{STRUCTURE_2oww| PDB=2oww | SCENE= }}
{{STRUCTURE_2oww| PDB=2oww | SCENE= }}
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'''Covalent intermediate in amylomaltase in complex with the acceptor analog 4-deoxyglucose'''
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===Covalent intermediate in amylomaltase in complex with the acceptor analog 4-deoxyglucose===
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==Overview==
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Amylomaltases are glycosyl hydrolases belonging to glycoside hydrolase family 77 that are capable of the synthesis of large cyclic glucans and the disproportionation of oligosaccharides. Using protein crystallography, we have generated a flip book movie of the amylomaltase catalytic cycle in atomic detail. The structures include a covalent glycosyl enzyme intermediate and a covalent intermediate in complex with an analogue of a co-substrate and show how the structures of both enzyme and substrate respond to the changes required by the catalytic cycle as it proceeds. Notably, the catalytic nucleophile changes conformation dramatically during the reaction. Also, Gln-256 on the 250s loop is involved in orienting the substrate in the +1 site. The absence of a suitable base in the covalent intermediate structure explains the low hydrolysis activity.
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The line below this paragraph, {{ABSTRACT_PUBMED_17420245}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17420245 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17420245}}
==About this Structure==
==About this Structure==
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[[Category: Beta-alpha barrel]]
[[Category: Beta-alpha barrel]]
[[Category: Covalent intermediate]]
[[Category: Covalent intermediate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:50:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:42:05 2008''

Revision as of 18:42, 28 July 2008

Image:2oww.png

Template:STRUCTURE 2oww

Covalent intermediate in amylomaltase in complex with the acceptor analog 4-deoxyglucose

Template:ABSTRACT PUBMED 17420245

About this Structure

2OWW is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Three-way stabilization of the covalent intermediate in amylomaltase, an alpha-amylase-like transglycosylase., Barends TR, Bultema JB, Kaper T, van der Maarel MJ, Dijkhuizen L, Dijkstra BW, J Biol Chem. 2007 Jun 8;282(23):17242-9. Epub 2007 Apr 9. PMID:17420245

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