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2p32

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{{STRUCTURE_2p32| PDB=2p32 | SCENE= }}
{{STRUCTURE_2p32| PDB=2p32 | SCENE= }}
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'''Crystal structure of the C-terminal 10 kDa subdomain from C. elegans Hsp70'''
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===Crystal structure of the C-terminal 10 kDa subdomain from C. elegans Hsp70===
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==Overview==
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Hsp70 chaperones are composed of two domains; the 40 kDa N-terminal nucleotide-binding domain (NDB) and the 30 kDa C-terminal substrate-binding domain (SBD). Structures of the SBD from Escherichia coli homologues DnaK and HscA show it can be further divided into an 18 kDa beta-sandwich subdomain, which forms the hydrophobic binding pocket, and a 10 kDa C-terminal three-helix bundle that forms a lid over the binding pocket. Across prokaryotes and eukaryotes, the NBD and beta-sandwich subdomain are well conserved in both sequence and structure. The C-terminal subdomain is, however, more evolutionary variable and the only eukaryotic structure from rat Hsc70 revealed a diverged helix-loop-helix fold. We have solved the crystal structure of the C-terminal 10 kDa subdomain from Caenorhabditis elegans Hsp70 which forms a helical-bundle similar to the prokaryotic homologues. This provides the first confirmation of the structural conservation of this subdomain in eukaryotes. Comparison with the rat structure reveals a domain-swap dimerisation mechanism; however, the C. elegans subdomain exists exclusively as a monomer in solution in agreement with the hypothesis that regions out with the C-terminal subdomain are necessary for Hsp70 self-association.
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(as it appears on PubMed at http://www.pubmed.gov), where 17407764 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17407764}}
==About this Structure==
==About this Structure==
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[[Category: Worrall, L J.]]
[[Category: Worrall, L J.]]
[[Category: Three-helix bundle]]
[[Category: Three-helix bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:14:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:51:34 2008''

Revision as of 12:51, 28 July 2008

Image:2p32.png

Template:STRUCTURE 2p32

Crystal structure of the C-terminal 10 kDa subdomain from C. elegans Hsp70

Template:ABSTRACT PUBMED 17407764

About this Structure

2P32 is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.

Reference

Crystal structure of the C-terminal three-helix bundle subdomain of C. elegans Hsp70., Worrall LJ, Walkinshaw MD, Biochem Biophys Res Commun. 2007 May 25;357(1):105-10. Epub 2007 Mar 28. PMID:17407764

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