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2p4v

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{{STRUCTURE_2p4v| PDB=2p4v | SCENE= }}
{{STRUCTURE_2p4v| PDB=2p4v | SCENE= }}
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'''Crystal structure of the transcript cleavage factor, GreB at 2.6A resolution'''
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===Crystal structure of the transcript cleavage factor, GreB at 2.6A resolution===
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==Overview==
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Bacterial Gre transcript cleavage factors stimulate the intrinsic endonucleolytic activity of RNA polymerase (RNAP) to rescue stalled transcription complexes. They bind to RNAP and extend their coiled-coil (CC) domains to the catalytic centre through the secondary channel. Three existing models for the Gre-RNAP complex postulate congruent mechanisms of Gre-assisted catalysis, while offering conflicting views of the Gre-RNAP interactions. Here, we report the GreB structure of Escherichia coli. The GreB monomers form a triangle with the tip of the amino-terminal CC of one molecule trapped within the hydrophobic cavity of the carboxy-terminal domain of a second molecule. This arrangement suggests an analogous model for recruitment to RNAP. Indeed, the beta'-subunit CC located at the rim of the secondary channel has conserved hydrophobic residues at its tip. We show that substitutions of these residues and those in the GreB C-terminal domain cavity confer defects in GreB activity and binding to RNAP, and present a plausible model for the RNAP-GreB complex.
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(as it appears on PubMed at http://www.pubmed.gov), where 17917675 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17917675}}
==About this Structure==
==About this Structure==
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[[Category: Transcript cleavage]]
[[Category: Transcript cleavage]]
[[Category: Transcription]]
[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:21:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:57:03 2008''

Revision as of 07:57, 29 July 2008

Image:2p4v.png

Template:STRUCTURE 2p4v

Crystal structure of the transcript cleavage factor, GreB at 2.6A resolution

Template:ABSTRACT PUBMED 17917675

About this Structure

2P4V is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The carboxy-terminal coiled-coil of the RNA polymerase beta'-subunit is the main binding site for Gre factors., Vassylyeva MN, Svetlov V, Dearborn AD, Klyuyev S, Artsimovitch I, Vassylyev DG, EMBO Rep. 2007 Nov;8(11):1038-43. Epub 2007 Oct 5. PMID:17917675

Page seeded by OCA on Tue Jul 29 10:57:03 2008

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