1fl3
From Proteopedia
(New page: 200px<br /> <applet load="1fl3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fl3, resolution 2.45Å" /> '''CRYSTAL STRUCTURE O...) |
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caption="1fl3, resolution 2.45Å" /> | caption="1fl3, resolution 2.45Å" /> | ||
'''CRYSTAL STRUCTURE OF THE BLUE FLUORESCENT ANTIBODY (19G2) IN COMPLEX WITH STILBENE HAPTEN AT 277K'''<br /> | '''CRYSTAL STRUCTURE OF THE BLUE FLUORESCENT ANTIBODY (19G2) IN COMPLEX WITH STILBENE HAPTEN AT 277K'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1FL3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SPB as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1FL3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=SPB:'>SPB</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FL3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: immunoglobulin fold]] | [[Category: immunoglobulin fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:47:59 2008'' |
Revision as of 13:47, 15 February 2008
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CRYSTAL STRUCTURE OF THE BLUE FLUORESCENT ANTIBODY (19G2) IN COMPLEX WITH STILBENE HAPTEN AT 277K
Overview
The forte of catalytic antibodies has resided in the control of the, ground-state reaction coordinate. A principle and method are now described, in which antibodies can direct the outcome of photophysical and, photochemical events that take place on excited-state potential energy, surfaces. The key component is a chemically reactive optical sensor that, provides a direct report of the dynamic interplay between protein and, ligand at the active site. To illustrate the concept, we used a, trans-stilbene hapten to elicit a panel of monoclonal antibodies that, displayed a range of fluorescent spectral behavior when bound to a, trans-stilbene substrate. Several antibodies yielded a blue fluorescence, indicative of an excited-state complex or "exciplex" between, trans-stilbene and the antibody. The antibodies controlled the, isomerization coordinate of trans-stilbene and dynamically coupled this, manifold with an active-site residue. A step was taken toward the use of, antibody-based photochemical sensors for diagnostic and clinical, applications.
About this Structure
1FL3 is a Protein complex structure of sequences from Mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
Blue-fluorescent antibodies., Simeonov A, Matsushita M, Juban EA, Thompson EH, Hoffman TZ, Beuscher AE 4th, Taylor MJ, Wirsching P, Rettig W, McCusker JK, Stevens RC, Millar DP, Schultz PG, Lerner RA, Janda KD, Science. 2000 Oct 13;290(5490):307-13. PMID:11030644
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