1jgu
From Proteopedia
(New page: 200px<br /> <applet load="1jgu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jgu, resolution 1.8Å" /> '''STRUCTURAL BASIS FOR...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1jgu. | + | [[Image:1jgu.jpg|left|200px]]<br /><applet load="1jgu" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1jgu" size=" | + | |
caption="1jgu, resolution 1.8Å" /> | caption="1jgu, resolution 1.8Å" /> | ||
'''STRUCTURAL BASIS FOR DISFAVORED ELIMINATION REACTION IN CATALYTIC ANTIBODY 1D4'''<br /> | '''STRUCTURAL BASIS FOR DISFAVORED ELIMINATION REACTION IN CATALYTIC ANTIBODY 1D4'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1JGU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with OH and HBC as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1JGU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=OH:'>OH</scene> and <scene name='pdbligand=HBC:'>HBC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JGU OCA]. |
==Reference== | ==Reference== | ||
| Line 24: | Line 23: | ||
[[Category: igg fold]] | [[Category: igg fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:07:18 2008'' |
Revision as of 14:07, 15 February 2008
|
STRUCTURAL BASIS FOR DISFAVORED ELIMINATION REACTION IN CATALYTIC ANTIBODY 1D4
Overview
Murine antibody 1D4 selectively catalyzes a highly disfavored, beta-elimination reaction. Crystal structures of unliganded 1D4 and 1D4 in, complex with a transition-state analog (TSA) have elucidated a possible, general base mode of catalysis. The structures of the unliganded and, liganded Fabs were determined to 1.80 and 1.85 A resolution, respectively., The structure of the complex reveals a binding pocket with high shape, complementarity to the TSA, which is recruited to coerce the substrate, into the sterically demanding, eclipsed conformation that is required for, catalysis. A histidine residue and two water molecules are likely involved, in the catalysis. The structure supports either a concerted E2 or stepwise, E1cB-like mechanism for elimination. Finally, the liganded 1D4 structure, shows minor conformational rearrangements in CDR H2, indicative of, induced-fit binding of the hapten. 1D4 has pushed the boundaries of, antibody-mediated catalysis into the realm of disfavored reactions and, hence, represents an important milestone in the development of this, technology.
About this Structure
1JGU is a Single protein structure of sequence from Mus musculus with and as ligands. Full crystallographic information is available from OCA.
Reference
Structural basis for a disfavored elimination reaction in catalytic antibody 1D4., Larsen NA, Heine A, Crane L, Cravatt BF, Lerner RA, Wilson IA, J Mol Biol. 2001 Nov 16;314(1):93-102. PMID:11724535
Page seeded by OCA on Fri Feb 15 16:07:18 2008
Categories: Mus musculus | Single protein | Crane, L. | Cravatt, B.F. | Heine, A. | Larsen, N.A. | Lerner, R.A. | Wilson, I.A. | HBC | OH | Igg fold
